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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 193 - 204 (12page)
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Sixteen acetic acid bacteria (AAB) were isolated from blueberries and citric fruits of the Salto Grande region (Concordia, Entre Ríos, Argentina) using enrichment techniques and plate isolation. Enrichment broths containing ethanol and acetic acid enabled maximum AAB recovery, since these components promote their growth. Biochemical tests allowed classification of the bacteria at genus level. PCR-RFLP of the 16S rRNA and PCR-RFLP of the 16S-23S rRNA intergenic spacer allowed further classification at the species level; this required treatment of the amplified products of 16S and 16S-23S ITS ribosomal genes with the following restriction enzymes: AluI, RsaI, HaeIII, MspI, TaqI, CfoI, and Tru9I. C7, C8, A80, A160, and A180 isolates were identified as Gluconobacter frateurii; C1, C2, C3, C4, C5, C6, A70, and A210 isolates as Acetobacter pasteurianus; A50 and A140 isolates as Acetobacter tropicalis; and C9 isolate as Acetobacter syzygii. The bacteria identified by 16S rRNA PCR-RFLP were validated by 16S-23S PCR-RFLP; however, the C1 isolate showed different restriction patterns during identification and validation. Partial sequencing of the 16S gene resolved the discrepancy.
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참고문헌 (42)
참고문헌 신청
Andrés-Barrao C / 2017 / Acetic acid bacteria fundamentals and food applications / CRC Press
Mateo E / 2014 / Acetic acid bacteria isolated from grapes of South Australian vineyards / Int. J. Food Microbiol 178:98~106
Lisdiyanti P / 2003 / Diversity of acetic acid bacteria in Indonesia, Thailand, and the Philippines / Microbiol. Cult Coll 19:91~99
Komagata K / 2014 / The Prokaryotes - Alphaproteobacteria and Betaproteobacteria / Springer-Verlag :547~577
Trček J / 2015 / Updates on quick identification of acetic acid bacteria with a focus on the 16S-23S rRNA gene internal transcribed spacer and the analysis of cell proteins by MALDITOF mass spectrometry / Int. J. Food Microbiol 196:137~144
Mateo E / 2014 / Acetic acid bacteria isolated from grapes of South Australian vineyards / Int. J. Food Microbiol 178:98~106
Lisdiyanti P / 2003 / Diversity of acetic acid bacteria in Indonesia, Thailand, and the Philippines / Microbiol. Cult Coll 19:91~99
Komagata K / 2014 / The Prokaryotes - Alphaproteobacteria and Betaproteobacteria / Springer-Verlag :547~577
Trček J / 2015 / Updates on quick identification of acetic acid bacteria with a focus on the 16S-23S rRNA gene internal transcribed spacer and the analysis of cell proteins by MALDITOF mass spectrometry / Int. J. Food Microbiol 196:137~144
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