지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한약리학회 The Korean Journal of Physiology & Pharmacology The Korean Journal of Physiology & Pharmacology 제24권 제3호
- 발행연도
- 2020.1
- 수록면
- 241 - 248 (8page)
이용수
초록· 키워드
오류제보하기
Luminespib (AUY922), a heat shock proteins 90 inhibitor, has anti-neoplastic and antitumor effects. However, it is not clear whether AUY922 affects events in vascular diseases. We investigated the effects of AUY922 on the platelet-derived growth factor (PDGF)-BB-stimulated proliferation and migration of vascular smooth muscle cells (VSMC). VSMC viability was detected using the XTT (2,3-bis-(2-methoxy- 4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) reagent. To detect the attenuating effects of AUY922 on PDGF-BB-induced VSMCs migration in vitro, we performed the Boyden chamber and scratch wound healing assays. To identify AUY922- mediated changes in the signaling pathway, the phosphorylation of protein kinase B (Akt) and extracellular signal-regulated kinase (ERK) 1/2 was analyzed by immunoblotting. The inhibitory effects of AUY922 on migration and proliferation ex vivo were tested using an aortic ring assay. AUY922 was not cytotoxic at concentrations up to 5 nM. PDGF-BB-induced VSMC proliferation, migration, and sprout outgrowth were significantly decreased by AUY922 in a dose-dependent manner. AUY922 significantly reduced the PDGF-BB-stimulated phosphorylation of Akt and ERK1/2. Furthermore, PD98059 (a selective ERK1/2 inhibitor) and LY294002 (a selective Akt inhibitor) decreased VSMC migration and proliferation by inhibiting phosphorylation of Akt and ERK1/2. Greater attenuation of PDGF-BB-induced cell viability and migration was observed upon treatment with PD98059 or LY294002 in combination with AUY922. AUY922 showed anti-proliferation and anti-migration effects towards PDGF-BBinduced VSMCs by regulating the phosphorylation of ERK1/2 and Akt. Thus, AUY922 is a candidate for the treatment of atherosclerosis and restenosis.
목차
등록된 정보가 없습니다.
참고문헌 (28)
참고문헌 신청
Frostegård J / 2013 / Immunity, atherosclerosis and cardiovascular disease / BMC Med 11:117
Tousoulis D / 2008 / Endothelial function and inflammation in coronary artery disease / Postgrad Med J 84:368~371
Chaabane C / 2013 / Biological responses in stented arteries / Cardiovasc Res 99:353~363
Won KJ / 2013 / DJ-1/park7 protects against neointimal formation via the inhibition of vascular smooth muscle cell growth / Cardiovasc Res 97:553~561
Casscells W / 1991 / Smooth muscle cell growth factors / Prog Growth Factor Res 3:177~206
Tousoulis D / 2008 / Endothelial function and inflammation in coronary artery disease / Postgrad Med J 84:368~371
Chaabane C / 2013 / Biological responses in stented arteries / Cardiovasc Res 99:353~363
Won KJ / 2013 / DJ-1/park7 protects against neointimal formation via the inhibition of vascular smooth muscle cell growth / Cardiovasc Res 97:553~561
Casscells W / 1991 / Smooth muscle cell growth factors / Prog Growth Factor Res 3:177~206
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
DE21 inhibits PDGF-induced proliferation of rat aortic vascular smooth muscle cell by up-regulation of p53 leading to G1/S cell cycle arrest
한국실험동물학회 학술발표대회 논문집
2015 .02
-
Glucagon-Like Peptide Receptor Agonist Inhibits Angiotensin II-Induced Proliferation and Migration in Vascular Smooth Muscle Cells and Ameliorates Phosphate-Induced Vascular Smooth Muscle Cells Calcification
Diabetes and Metabolism Journal
2024 .01
-
S1P<sub>1</sub> Regulates M1/M2 Polarization toward Brain Injury after Transient Focal Cerebral Ischemia
Biomolecules & Therapeutics(구 응용약물학회지)
2019 .01
-
DN200434 Inhibits Vascular Smooth Muscle Cell Proliferation and Prevents Neointima Formation in Mice after Carotid Artery Ligation
Endocrinology and Metabolism
2022 .10
-
인체 모유두세포의 증식에 미치는 갈근 에탄올추출물의 효과
동의생리병리학회지
2017 .06
-
인체 모유두세포의 증식에 미치는 갈근 에탄올추출물의 효과
동의생리병리학회지
2017 .01
-
Murrayafoline A Induces a G0/G1-Phase Arrest in Platelet-Derived Growth Factor-Stimulated Vascular Smooth Muscle Cells
The Korean Journal of Physiology & Pharmacology
2015 .09
-
Gallic Acid Inhibits Proliferation and Migration of Smooth Muscle Cells in a Pig In-Stent Restenosis Model
전남의대학술지
2024 .01
-
Anti-apoptotic effects of autophagy via ROS regulation in microtubule-targeted and PDGF-stimulated vascular smooth muscle cells
The Korean Journal of Physiology & Pharmacology
2018 .05
-
Protective effect and mechanism of ginsenoside Rg2 on atherosclerosis
Journal of Ginseng Research
2023 .03
-
Anti-Proliferative Effects of Rutin on OLETF Rat Vascular Smooth Muscle Cells Stimulated by Glucose Variability
Yonsei Medical Journal
2016 .01
-
Integrin αvβ3 Induces HSP90 Inhibitor Resistance via FAK Activation in KRAS-Mutant Non-Small Cell Lung Cancer
Cancer Research and Treatment
2022 .07
-
Therapeutic targets and drugs for hyper-proliferation of vascular smooth muscle cells
Journal of Pharmaceutical Investigation
2020 .01
-
저칼륨혈증 흰쥐 신장에서 Akt, p-Akt, ERK 및 p-ERK 단백발현의 변화
해부·생물인류학
2017 .09
-
SD-317 inhibits cell growth and proliferation by preventing activation of Akt in lung cancer
한국실험동물학회 학술발표대회 논문집
2020 .07
-
The proper concentrations of dextrose and lidocaine in regenerative injection therapy: in vitro study
The Korean Journal of Pain
2021 .01
-
Fluvastatin inhibits advanced glycation end products-induced proliferation, migration, and extracellular matrix accumulation in vascular smooth muscle cells by targeting connective tissue growth factor
The Korean Journal of Physiology & Pharmacology
2018 .03
-
Effects of Homocysteine and Hyperglycemia on the Proliferation of Aortic Vascular Smooth Muscle Cells of Obese Type 2 Diabetes Rat
Annals of geriatric medicine and research
2017 .01
-
Inhibitory effect of ginsenglactone A from Panax ginseng on the tube formation of human umbilical vein endothelial cells and migration of human ovarian cancer cells
Journal of Ginseng Research
2023 .03
이 논문의 저자 정보
최근 본 자료
댓글(0)
0