지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2023.3
- 수록면
- 246 - 254 (9page)
이용수
초록· 키워드
오류제보하기
Background: Here, we aimed to assess the inhibitory effect of a new compound from Panax ginseng on the migration of human ovarian cancer cells and tube formation of human umbilical vein endothelial cells (HUVECs).
Methods: A new compound, ginsenglactone A (1), was isolated from ginseng roots, together with seven known compounds (2-8). Spectroscopic data were used to elucidate the chemical structure of 1. The tubular structure formation in HUVECs was assessed by Mayer"s hematoxylin staining. The migration of A2780 cells was evaluated using the scratch wound healing assay.
Results: HUVECs treated with 1 had the statistically significant decrease in tubular structure formation compared to the HUVECs treated with compounds 2-8. This effect was enhanced by co-treatment with inhibitors for phosphatidylinositol 3-kinase (PI3K) (LY294002) and extracellular signal-regulated kinase (ERK) (U0126). Treatment with 1 decreased the expression of phosphorylation of ERK, PI3K, vascular endothelial growth factor receptor2 (VEGFR2), Akt, and mammalian target of rapamycin (mTOR). In addition, the ability of A2780 cells to cover the scratched area were also decreased. This effect was enhanced by co-treatment with U0126. Lastly, treatment with 1 decreased the phosphorylation of ERK, matrix metalloproteinase-9 (MMP-9), and MMP-2.
Conclusion: These results suggest that ginsenglactone A is a potential inhibitor of HUVEC tubular structure formation and A2780 cellular migration, which may be helpful for understanding its anticancer mechanism.
Methods: A new compound, ginsenglactone A (1), was isolated from ginseng roots, together with seven known compounds (2-8). Spectroscopic data were used to elucidate the chemical structure of 1. The tubular structure formation in HUVECs was assessed by Mayer"s hematoxylin staining. The migration of A2780 cells was evaluated using the scratch wound healing assay.
Results: HUVECs treated with 1 had the statistically significant decrease in tubular structure formation compared to the HUVECs treated with compounds 2-8. This effect was enhanced by co-treatment with inhibitors for phosphatidylinositol 3-kinase (PI3K) (LY294002) and extracellular signal-regulated kinase (ERK) (U0126). Treatment with 1 decreased the expression of phosphorylation of ERK, PI3K, vascular endothelial growth factor receptor2 (VEGFR2), Akt, and mammalian target of rapamycin (mTOR). In addition, the ability of A2780 cells to cover the scratched area were also decreased. This effect was enhanced by co-treatment with U0126. Lastly, treatment with 1 decreased the phosphorylation of ERK, matrix metalloproteinase-9 (MMP-9), and MMP-2.
Conclusion: These results suggest that ginsenglactone A is a potential inhibitor of HUVEC tubular structure formation and A2780 cellular migration, which may be helpful for understanding its anticancer mechanism.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
5. Conclusions
References
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Effects of gintonin on the proliferation, migration, and tube formation of human umbilical-vein endothelial cells : involvement of lysophosphatidic-acid receptors and vascular-endothelial-growth-factor signaling
Journal of Ginseng Research
2016 .10
-
Myricetin Inhibits Angiogenesis by Inducing Apoptosis and Suppressing PI3K/Akt/mTOR Signaling in Endothelial Cells
대한암예방학회지
2017 .01
-
Antiangiogenic Effect of Ethanol Extract of Vigna angularisvia Inhibition of Phosphorylation of VEGFR2, Erk, and Akt
대한동의병리학회 학술대회논문집
2015 .10
-
Effects of fermented black ginseng on wound healing mediated by angiogenesis through the mitogen-activated protein kinase pathway in human umbilical vein endothelial cells
Journal of Ginseng Research
2018 .10
-
Propofol attenuates hydrogenperoxide- induced apoptosis in human umbilical vein endothelial cells via multiple signaling pathways
Korean Journal of Anesthesiology
2015 .01
-
Galpha12 Protects Vascular Endothelial Cells from Serum Withdrawal-Induced Apoptosis through Regulation of miR-155
Yonsei Medical Journal
2016 .01
-
Functional role of ginseng-derived compounds in cancer
Journal of Ginseng Research
2018 .06
-
Korean Red ginseng prevents endothelial senescence by downregulating the HO-1/NF-κB/miRNA-155-5p/eNOS pathway
Journal of Ginseng Research
2021 .03
-
Adipose Stromal Cells from Visceral and Subcutaneous Fat Facilitate Migration of Ovarian Cancer Cells via IL-6/JAK2/STAT3 Pathway
Cancer Research and Treatment
2017 .01
-
Ginsenoside compound K protects human umbilical vein endothelial cells against oxidized low-density lipoprotein-induced injury via inhibition of nuclear factor-kB, p38, and JNK MAPK pathways
Journal of Ginseng Research
2019 .01
-
GPR40 Agonism Modulates Inflammatory Reactions in Vascular Endothelial Cells
Diabetes and Metabolism Journal
2022 .05
-
Zerumbone, Sesquiterpene Photochemical from Ginger, Inhibits Angiogenesis
The Korean Journal of Physiology & Pharmacology
2015 .07
-
4-Hexylresorcinol-in duced angiogenesis potential in human endothelial cells
Maxillofacial Plastic Reconstructive Surgery
2020 .01
-
Advanced tube formation assay using human endothelial colony forming cells for in vitro evaluation of angiogenesis
The Korean Journal of Physiology & Pharmacology
2018 .11
-
고려인삼(Panax ginseng)과 미국삼(Panax quinquefolium)의 승열작용에 대한 비교 고찰
생약학회지(Korean Journal of Pharmacognosy)
2018 .09
-
Increase in apoptotic effect of Panax ginseng by microwave processing in human prostate cancer cells : in vitro and in vivo studies
Journal of Ginseng Research
2016 .01
-
Systems-level mechanisms of action of Panax ginseng: a network pharmacological approach
Journal of Ginseng Research
2018 .01
-
청혈플러스가 혈관내피세포에서 염증 지표인자에 미치는 영향
혜화의학회지
2018 .01
-
Panax ginseng total protein promotes proliferation and secretion of collagen in NIH/3T3 cells by activating extracellular signal-related kinase pathway
Journal of Ginseng Research
2017 .07
-
5637 인체 방광암세포의 이동성과 침윤성에 미치는 황흑산(黃黑散)의 영향
대한한방내과학회지
2016 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2023-524-001465819