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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한약리학회 The Korean Journal of Physiology & Pharmacology The Korean Journal of Physiology & Pharmacology 제22권 제6호
- 발행연도
- 2018.11
- 수록면
- 705 - 712 (8page)
- DOI
- https://doi.org/10.4196/kjpp.2018.22.6.705
이용수
초록· 키워드
오류제보하기
The tube formation assay is a widely used in vitro experiment model to evaluate angiogenic properties by measuring the formation of tubular structures from vascular endothelial cells (ECs). In vitro experimental results are crucial when considered the advisability of moving forward to in vivo studies. Thus, the additional attentions to the in vitro assay is necessary to improve the quality of the pre-clinical data, leading to better decision-making for successful drug discovery. In this study, we improved the tube formation assay system in three aspects. First, we used human endothelial colony forming cells (ECFCs), which are endothelial precursors that have a robust proliferative capacity and more defined angiogenic characteristics compared to mature ECs. Second, we utilized a real-time cell recorder to track the progression of tube formation for 48 hours. Third, to minimize analysis error due to the limited observation area, we used image-stitching software to increase the microscope field of view to a 2×2 stitched area from the 4× object lens. Our advanced tube formation assay system successfully demonstrated the time-dependent dynamic progression of tube formation in the presence and absence of VEGF and FGF-2. Vatalanib, VEGF inhibitor, was tested by our assay system. Of note, IC50 values of vatalanib was different at each observation time point. Collectively, these results indicate that our advanced tube formation assay system replicates the dynamic progression of tube formation in response to angiogenic modulators. Therefore, this new system provides a sensitive and versatile assay model for evaluating pro- or anti-angiogenic drugs.
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참고문헌 (2)
참고문헌 신청
김미경 / 2015 / Involvement of Heme Oxygenase-1 in Orexin-A-induced Angiogenesis in Vascular Endothelial Cells / The Korean Journal of Physiology & Pharmacology 19(4):327~334
김다연 / 2018 / Hypoxia-dependent mitochondrial fission regulates endothelial progenitor cell migration, invasion, and tube formation / The Korean Journal of Physiology & Pharmacology 22(2):203~213
김다연 / 2018 / Hypoxia-dependent mitochondrial fission regulates endothelial progenitor cell migration, invasion, and tube formation / The Korean Journal of Physiology & Pharmacology 22(2):203~213
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