지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.7
- 수록면
- 411 - 418 (8page)
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초록· 키워드
오류제보하기
Background: Recently, protein from ginseng was studied and used for the treatment of several kinds of diseases. However, the effect of ginseng total protein (GTP) on proliferation and wound healing in fibroblast cells remains unclear.
Methods: In this study, cell viability was analyzed using the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. Cell cycle distribution was analyzed by flow cytometer. The levels of transforming growth factor b1, vascular endothelial growth factor, and collagens were analyzed by enzyme-linked immunosorbent assay and immunofluorescence staining. The expressions of cyclin A, phosphorylation of extracellular signal-related kinase (p-ERK1/2), and ERK1/2 were analyzed byWestern blotting.
Results: Our results showed that GTP promoted cell proliferation and increased the percentage of cells in S phase through the upregulation of cyclin A in NIH/3T3 cells. We also found that GTP induced the secretion of type I collagen, and promoted the expression of other factors that regulate the synthesis of collagen such as transforming growth factor b1 and vascular endothelial growth factor. In addition, the phosphorylation of ERK1/2 at Thr202/Tyr204 was also increased by GTP.
Conclusion: Our studies suggest that GTP promoted proliferation and secretion of collagen in NIH/3T3 cells by activating the ERK signal pathway, which shed light on a potential function of GTP in promoting wound healing.
Methods: In this study, cell viability was analyzed using the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. Cell cycle distribution was analyzed by flow cytometer. The levels of transforming growth factor b1, vascular endothelial growth factor, and collagens were analyzed by enzyme-linked immunosorbent assay and immunofluorescence staining. The expressions of cyclin A, phosphorylation of extracellular signal-related kinase (p-ERK1/2), and ERK1/2 were analyzed byWestern blotting.
Results: Our results showed that GTP promoted cell proliferation and increased the percentage of cells in S phase through the upregulation of cyclin A in NIH/3T3 cells. We also found that GTP induced the secretion of type I collagen, and promoted the expression of other factors that regulate the synthesis of collagen such as transforming growth factor b1 and vascular endothelial growth factor. In addition, the phosphorylation of ERK1/2 at Thr202/Tyr204 was also increased by GTP.
Conclusion: Our studies suggest that GTP promoted proliferation and secretion of collagen in NIH/3T3 cells by activating the ERK signal pathway, which shed light on a potential function of GTP in promoting wound healing.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
References
참고문헌 (42)
참고문헌 신청
[학술지(정기간행물)] - Attele AS / 1999 / Ginseng pharmacology: multiple constituents and multiple actions / Biochem Pharmacol 58 : 85 ~ 93
[학술지(정기간행물)] - Chen X. / 1996 / Cardiovascular protection by ginsenosides and their nitric oxide releasing action / Clin Exp Pharmacol Physiol 23 : 728 ~ 732
[학술지(정기간행물)] - Subhasree Ashok Nag / 2012 / Ginsenosides as anticancer agents: In vitro and in vivo activities, structure–activity relationships, and molecular mechanisms of action / Frontiers in Pharmacology 3
[학술지(정기간행물)] - Young Gon Kim / 2008 / Effects of Red Ginseng extract on ultraviolet B-irradiated skin change in C57BL mice / Phytotherapy Research 22 (11) : 1423 ~ 1427
[학술지(정기간행물)] - Yoshiyuki Kimura / 2006 / Effects of ginseng saponins isolated from Red Ginseng roots on burn wound healing in mice / British Journal of Pharmacology 148 (6) : 860 ~ 870
[학술지(정기간행물)] - Chen X. / 1996 / Cardiovascular protection by ginsenosides and their nitric oxide releasing action / Clin Exp Pharmacol Physiol 23 : 728 ~ 732
[학술지(정기간행물)] - Subhasree Ashok Nag / 2012 / Ginsenosides as anticancer agents: In vitro and in vivo activities, structure–activity relationships, and molecular mechanisms of action / Frontiers in Pharmacology 3
[학술지(정기간행물)] - Young Gon Kim / 2008 / Effects of Red Ginseng extract on ultraviolet B-irradiated skin change in C57BL mice / Phytotherapy Research 22 (11) : 1423 ~ 1427
[학술지(정기간행물)] - Yoshiyuki Kimura / 2006 / Effects of ginseng saponins isolated from Red Ginseng roots on burn wound healing in mice / British Journal of Pharmacology 148 (6) : 860 ~ 870
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UCI(KEPA) : I410-ECN-0101-2018-524-001599140