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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한소아내분비학회 Annals of Pediatirc Endocrinology & Metabolism Annals of Pediatric Endocrinology & Metabolism 제26권 제4호
- 발행연도
- 2021.12
- 수록면
- 252 - 258 (7page)
- DOI
- 10.6065/apem.2040266.133
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초록· 키워드
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Purpose: Sphingosine kinase is a lipid kinase that phosphorylates sphingosine to generate sphingosine 1-phosphate (S1P). S1P regulates pancreatic islet β-cell endoplasmic reticulum stress and proliferation. Type 1 and type 2 diabetes share some key pathogenic processes. In this study, we investigated whether secretion of insulin and production of S1P is altered in alloxan and glucose-treated cells from the rat pancreatic β-cell line RIN-5F.Methods: RIN-5F cells were treated with 2 mM alloxan and 20 mM glucose for 6 hours or 24 hours before being evaluated by enzyme linked immunosorbent assay (ELISA) and Western blotting.Results: Insulin secretion and expression was higher in RIN-5F cells treated with glucose compared to control cells. In contrast, alloxan treatment did not affect insulin secretion and expression in RIN-5F cells. Interestingly, compared with normal control levels, S1P/EDG-5 was increased in both alloxan and glucose-treated pancreatic β cell than normal control. Mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) inhibition strongly decreased the expression of insulin and S1P in glucose- or alloxan-treated RIN-5F cells.Conclusion: We observe that production of S1P is increased in both diabetic cell models. In addition, MAPK/ERK signaling regulates secretion of insulin and S1P expression in pancreatic β-cells. Based on the literature and our findings, S1P may be a promising agent for the treatment of insulin-related disorders.
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참고문헌 (26)
참고문헌 신청
Craig ME / 2014 / ISPAD Clinical Practice Consensus Guidelines 2014. Definition, epidemiology, and classification of diabetes in children and adolescents / Pediatr Diabetes 15(Suppl 20):4~17
White MF / 2003 / Insulin signaling in health and disease / Science 302:1710~1711
Pinhas-Hamiel O / 2007 / Acute and chronic complications of type 2 diabetes mellitus in children and adolescents / Lancet 369:1823~1831
Craig ME / 2007 / Diabetes care, glycemic control, and complications in children with type 1 diabetes from Asia and the Western Pacific Region / J Diabetes Complications 21:280~287
Saini V / 2010 / Molecular mechanisms of insulin resistance in type 2 diabetes mellitus / World J Diabetes 1:68~75
White MF / 2003 / Insulin signaling in health and disease / Science 302:1710~1711
Pinhas-Hamiel O / 2007 / Acute and chronic complications of type 2 diabetes mellitus in children and adolescents / Lancet 369:1823~1831
Craig ME / 2007 / Diabetes care, glycemic control, and complications in children with type 1 diabetes from Asia and the Western Pacific Region / J Diabetes Complications 21:280~287
Saini V / 2010 / Molecular mechanisms of insulin resistance in type 2 diabetes mellitus / World J Diabetes 1:68~75
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