Response of Glucocorticoid Receptor Alpha and Histone Deacetylase 2 to Glucocorticoid Treatment Predicts the Prognosis of Sudden Sensorineural Hearing Loss

Xiuling Zhang; Jinxiang Chen; Ziwen Gao; Hui Qi; Yanhong Dai; Wandong She

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자료유형: 학술저널

저자정보: Xiuling Zhang Jinxiang Chen Ziwen Gao Hui Qi Yanhong Dai Wandong She

저널정보: 대한이비인후과학회 Clinical and Experimental Otorhinolaryngology Clinical and Experimental Otorhinolaryngology 제12권 제4호

발행연도: 2019.1

수록면: 367 - 375 (9page)

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Objectives. To investigate glucocorticoid receptor (GR) and histone deacetylase 2 (HDAC2) gene expression and protein levels in peripheral blood mononuclear cells (PBMCs) of patients with severe or profound sudden sensorineural hearing loss (SSNHL) and to explore the roles of GRs and HDAC2 in glucocorticoid (GC) insensitivity. Methods. Fifty-five severe or profound SSNHL patients were enrolled in the study. According to hearing improvement after GC treatment, patients were assigned into two groups: GC-sensitive and GC-resistant. A normal reference group included 20 healthy volunteers without hearing loss. Quantitative real-time polymerase chain reaction and Western blot analyses were used to detect the relative expression of GRα, GRβ, and HDAC2 in PBMCs at the mRNA and protein levels. Results. The protein levels of GRs and HDAC2 in PBMCs of SSNHL patients were lower than the normal reference values before GC treatment. Compared with the GC-resistant group, both the mRNA and protein levels of GRα and HDAC2 were significantly increased in the GC-sensitive group after GC treatment. Conclusion. A lack of GRα and HDAC2 induction following steroid treatment in GC-resistant SSNHL patients may play a fundamental mechanistic role in GC insensitivity. Response of GRα and HDAC2 to steroid treatment may, thus, predict the prognosis of hearing improvement in SSNHL patients.

참고문헌 (27)

참고문헌 신청

El Sabbagh NG / 2017 / Intratympanic dexamethasone in sudden sensorineural hearing loss : a systematic review and meta-analysis / Laryngoscope 127(8):1897~1908

Trune DR / 2012 / Corticosteroid therapy for hearing and balance disorders / Anat Rec(Hoboken) 295(11):1928~1943

Stachler RJ / 2012 / Clinical practice guideline: sudden hearing loss / Otolaryngol Head Neck Surg 146(3 Suppl):S1~S35

Michel O / 2011 / The revised version of the German guidelines"sudden idiopathic sensorineural hearing loss" / Laryngorhinootologie 90(5):290~293

Gul F / 2017 / A comprehensive study of oxidative stress in sudden hearing loss / Eur Arch Otorhinolaryngol 274(3):1301~1308

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