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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 509 - 516 (8page)
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Purpose: This study was conducted to verify the induction and mechanism of selective apoptosis in G361 melanoma cells usinganti-HER2 antibody-conjugated gold nanoparticles (GNP-HER2). Materials and Methods: Following GNP-HER2 treatment of G361 cells, cell cycle arrest and apoptosis were measured by WST-1assay, Hemacolor staining, Hoechst staining, immunofluorescence staining, fluorescence-activated cell sorting analysis, andWestern blotting. Results: G361 cells treated with GNP-HER2 showed condensation of nuclei, which is an apoptotic phenomenon, and translocationof apoptosis-inducing factor and cytochrome c from mitochondria into the nucleus and cytoplasm, respectively. Increases inBAX in cells undergoing apoptosis, activation of caspase-3 and -9, and fragmentation of poly (ADP-ribose) polymerase and DNAfragmentation factor 45 (inhibitor of caspase-activated DNase) were observed upon GNP-HER2 treatment. Following GNP-HER2treatment, an increase of cells in sub-G1 phase, which is a signal of cell apoptosis, was observed. This resulted in the down-regulationof cyclin A, cyclin D1, cyclin E, cdk2, cdk4, and cdc2 and the up-regulation of p21. Thus, GNP-HER2 treatment was confirmedto induce the cessation of cell cycle progression. Also, decreases in phospho-focal adhesion kinase and phospho-humanepidermal growth factor receptor, which activate cellular focal adhesion, and decreases in phospho-paxillin, which stimulates thedisassembly of filamentous actin, were observed. Reduced cell adhesion and disassembly of the intracellular structure indicatedcell deactivation. Conclusion: GNP-HER2 can selectively kill G361 melanoma cells without affecting normal cells. The mechanism of G361 celldeath upon treatment with GNP-HER2 was apoptosis accompanied by activation of caspases.
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참고문헌 (32)
참고문헌 신청
Kim SH / 2016 / Microsomal PGE2 synthase-1 regulates melanoma cell survival and associates with melanoma disease progression / Pigment Cell Melanoma Res 29:297~308
Soengas MS / 2003 / Apoptosis and melanoma chemoresistance / Oncogene 22:3138~3151
Service RF / 2005 / Materials and biology. Nanotechnology takes aim at cancer / Science 310:1132~1134
Lam CW / 2004 / Pulmonary toxicity of single-wall carbon nanotubes in mice 7 and 90 days after intratracheal instillation / Toxicol Sci 77:126~134
Shi Kam NW / 2004 / Nanotube molecular transporters : internalization of carbon nanotube-protein conjugates into Mammalian cells / J Am Chem Soc 126:6850~6851
Soengas MS / 2003 / Apoptosis and melanoma chemoresistance / Oncogene 22:3138~3151
Service RF / 2005 / Materials and biology. Nanotechnology takes aim at cancer / Science 310:1132~1134
Lam CW / 2004 / Pulmonary toxicity of single-wall carbon nanotubes in mice 7 and 90 days after intratracheal instillation / Toxicol Sci 77:126~134
Shi Kam NW / 2004 / Nanotube molecular transporters : internalization of carbon nanotube-protein conjugates into Mammalian cells / J Am Chem Soc 126:6850~6851
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