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논문 기본 정보

자료유형
학술저널
저자정보
Oh, Eun-Ha (Environmental Toxico-Gemomic and Proteomic Center, School of Medicine, Korea University) Oh, Sang-Nam (Environmental Toxico-Gemomic and Proteomic Center, School of Medicine, Korea University) Im, Ho-Sub (School of Public Health, Korea University) Lee, Joo-Hyun (School of Public Health, Korea University) Kim, Jin-Young (School of Public Health, Korea University) Moon, Joo-Hee (School of Public Health, Korea University) Hong, Eun-Young (School of Public Health, Korea University) Kim, Yang-Hee (Environmental Toxico-Gemomic and Proteomic Center, School of Medicine, Korea University) Yang, Min-Ho (Environmental Toxico-Gemomic and Proteomic Center, School of Medicine, Korea University) Lim, Yong-Chul (Environmental Toxico-Gemomic and Proteomic Center, School of Medicine, Korea University) Park, Sun-Young (School of Public Health, Korea University) Lee, Eun-Il (Environmental Toxico-Gemomic and Proteomic Center, School of Medicine, Korea University) Sul, Dong-Geun (Environmental Toxico-Gemomic and Proteomic Center, School of Medicine, Korea University)
저널정보
대한독성유전단백체학회 Molecular & cellular toxicology Molecular & cellular toxicology 제1권 제2호
발행연도
2005.1
수록면
116 - 123 (8page)

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The application of high pressure on cellular morphology, proliferation and protein expression of Jurkat cells (human T lymphocyte cell line) has been extensively investigated. In the present study, we manufactured a novel pressure chamber that modulates 5% $CO_{2}$, temperature and pressure (up to 3 ATA). Jurkat cells was incubated 2 ATA pressure and analyzed cellular morphology and growth using an electron microscopy and MTT assay. The cells showed the morphological changes in the cell surface, which appeared to cause a severe damage in cell membrane. The growth rate of the cells under 2 ATA pressure decreased as cultured time got increased. Furthermore, a long term exposure of high pressure on Jurkat cells may act as one of the important cellular stresses that leads to inducing cell death. Cellular proteomes were separated by 2-dimensional electrophoresis with pH 3-10 ranges of IPG Dry strips. And many proteins showed significant up-and-down expressions with hyperbaric pressure. Out of all, 10 spots were identified significantly using matrix-assisted laser desorption/ionization-time of fight (MALDI-TOF) mass spectrometry. We and found that 9 protein expressions were decreased and one protein, heat shock protein HSP 60, was increased in Jurkat cells under 2 ATA. Identified proteins were related to lipid metabolism and signal transduction.

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