지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.10
- 수록면
- 225 - 237 (13page)
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초록· 키워드
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Purpose: FK506 (tacrolimus) is a widely used immunosuppressive agent in the treatment of various medical conditions, including autoimmune disease, bone marrow and organ transplantations. Previously FK506 was known to cause apoptotic death of human Jurkat T cells.
Methods: The current study was designed to analyze the gene expression pattern of Jurkat T cells after FK506 application by using cDNA microarray. Treatment of Jurkat T cells with FK506 resulted in a decrease of cell viability in a time- and dose-dependent manner. Next, total RNA of Jurkat T cells was extracted by using TRIzol reagent and used to carry out a confirmation test for the purity and integrity of total RNA.
Results: Gene expression levels related to apoptosis and cell cycle process were mainly focused to analyze in FK506-treated Jurkat T cells. According to the inhibition of calcineurin activity, MARCKS in PKC substrates and Sp3 transcription factor was markedly increased in FK506-treated cells. Also, cell cycle control gene Id1 and Id3 were induced in expression from FK506-treated Jurkat T cells. However, FK506 decreased the expression of Src homology 2, G protein, and MEK 2 genes in bioactive peptide induced signaling pathway. It also reduced the expression level of the insulin receptor, DRPLA and Bai1-associated protein 2 genes, which are involved in the regulation of cell motility and morphology control.
Conclusion: The author will continue to pursue the exact functional roles of genes that are markedly changed in expression by FK506 in human Jurkat T cells in vitro and in vivo experimental models.
Methods: The current study was designed to analyze the gene expression pattern of Jurkat T cells after FK506 application by using cDNA microarray. Treatment of Jurkat T cells with FK506 resulted in a decrease of cell viability in a time- and dose-dependent manner. Next, total RNA of Jurkat T cells was extracted by using TRIzol reagent and used to carry out a confirmation test for the purity and integrity of total RNA.
Results: Gene expression levels related to apoptosis and cell cycle process were mainly focused to analyze in FK506-treated Jurkat T cells. According to the inhibition of calcineurin activity, MARCKS in PKC substrates and Sp3 transcription factor was markedly increased in FK506-treated cells. Also, cell cycle control gene Id1 and Id3 were induced in expression from FK506-treated Jurkat T cells. However, FK506 decreased the expression of Src homology 2, G protein, and MEK 2 genes in bioactive peptide induced signaling pathway. It also reduced the expression level of the insulin receptor, DRPLA and Bai1-associated protein 2 genes, which are involved in the regulation of cell motility and morphology control.
Conclusion: The author will continue to pursue the exact functional roles of genes that are markedly changed in expression by FK506 in human Jurkat T cells in vitro and in vivo experimental models.
목차
서론
방법
결과
고찰
결론
REFERENCES
참고문헌 (26)
참고문헌 신청
1. [학술지(정기간행물)] - Pridohl O / 2001 / Low-dose immunosuppression with FK 506 and sirolimus after liver transplantation: 1-year results / Transplant Proc 33 : 3229 ~ 3231
2. [학술지(정기간행물)] - Harding MW / 1989 / A receptor for the immunosuppressant FK506 is a cis-trans peptidyl-prolyl isomerase / Nature 341 : 758 ~ 760
3. [학술지(정기간행물)] - Maki N / 1990 / Complementary DNA encoding the human T-cell FK506-binding protein, a peptidylprolyl cis-trans isomerase distinct from cyclophilin / Proc Natl Acad Sci U S A 87 : 5440 ~ 5443
4. [학술지(정기간행물)] - Brown EJ / 1994 / A mammalian protein targeted by G1-arresting rapamycin-receptor complex / Nature 369 : 756 ~ 758
5. [학술지(정기간행물)] - Bagnis C / 1997 / Comparative acute nephrotoxicity of FK-506 and ciclosporin in an isolated in situ autoperfused rat kidney model / Am J Nephrol 17 : 17 ~ 24
2. [학술지(정기간행물)] - Harding MW / 1989 / A receptor for the immunosuppressant FK506 is a cis-trans peptidyl-prolyl isomerase / Nature 341 : 758 ~ 760
3. [학술지(정기간행물)] - Maki N / 1990 / Complementary DNA encoding the human T-cell FK506-binding protein, a peptidylprolyl cis-trans isomerase distinct from cyclophilin / Proc Natl Acad Sci U S A 87 : 5440 ~ 5443
4. [학술지(정기간행물)] - Brown EJ / 1994 / A mammalian protein targeted by G1-arresting rapamycin-receptor complex / Nature 369 : 756 ~ 758
5. [학술지(정기간행물)] - Bagnis C / 1997 / Comparative acute nephrotoxicity of FK-506 and ciclosporin in an isolated in situ autoperfused rat kidney model / Am J Nephrol 17 : 17 ~ 24
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UCI(KEPA) : I410-ECN-0101-2013-514-002650491