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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.11
- 수록면
- 310 - 319 (10page)
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이 논문의 연구 히스토리 (3)
2009
Tacrolimus(FK506)에 의한 인간 Jurkat 림프구의 MicroRNA Profiling
이호균
,
최수진나
,
정상영
대한외과학회 학술대회 초록집
2009.11
학술대회자료
Tacrolimus (FK506)에 의한 인간 Jurkat 림프구의 MicroRNA Profiling
이호균
,
최수진나
,
정상영
대한외과학회 학술대회 초록집
2009.11
학술대회자료
FK506이 T 림프구 사멸에서 활성산소 생성에 미치는 영향
이호균
,
정상영
,
최수진나
Annals of Surgical Treatment and Research
2009.11
학술저널
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Purpose: Tacrolimus (FK506) has been widely used as an immunosuppressant in organ transplanted recipients to suppress organ rejection phenomenon. We investigated the role of oxidative stress and heme oxygense-1 by FK506 on human Jurkat T cells.
Methods: The cells viability was examined by DAPI stain, enzyme activity of caspase family proteins, and western blotting for Baks, PUMA, iNOS, HO-1. Cells were cultured in the absence or presence of CoPPIX or ZnPPIX and the fluorescence intensity was analyzed using a flow cytometry.
Results: Treatment with FK506 increased the generation of reactive oxygen species (ROS), including hydrogen peroxide and superoxide anion, and NO in Jurkat cells in a dose-dependent manner. Immunohistochemistry and Western blot analysis data revealed the hemoxygenase-1 (HO-1) was induced by the addition of FK506 in Jurkat cells. Induction of CoPP, HO-1 inducer, resulted in decreased intracellular H₂O₂ and NO concentrations. Instead ZnPP, an HO-1 competitive inhibitor did it reversely. In addition, ZnPP regulates iNOS protein synthesis by inhibition of HO-1.
Conclusion: Increase of HO-1 expression would induce to decrease the intracellular H₂O₂ and NO concentrations. Also, HO-1 would regulate iNOS protein synthesis. Consequently, we can expect the regulation of HO-1 expression with concomitants use of FK506 to suppress organ rejection phenomenon by enhancing apoptosis.
Methods: The cells viability was examined by DAPI stain, enzyme activity of caspase family proteins, and western blotting for Baks, PUMA, iNOS, HO-1. Cells were cultured in the absence or presence of CoPPIX or ZnPPIX and the fluorescence intensity was analyzed using a flow cytometry.
Results: Treatment with FK506 increased the generation of reactive oxygen species (ROS), including hydrogen peroxide and superoxide anion, and NO in Jurkat cells in a dose-dependent manner. Immunohistochemistry and Western blot analysis data revealed the hemoxygenase-1 (HO-1) was induced by the addition of FK506 in Jurkat cells. Induction of CoPP, HO-1 inducer, resulted in decreased intracellular H₂O₂ and NO concentrations. Instead ZnPP, an HO-1 competitive inhibitor did it reversely. In addition, ZnPP regulates iNOS protein synthesis by inhibition of HO-1.
Conclusion: Increase of HO-1 expression would induce to decrease the intracellular H₂O₂ and NO concentrations. Also, HO-1 would regulate iNOS protein synthesis. Consequently, we can expect the regulation of HO-1 expression with concomitants use of FK506 to suppress organ rejection phenomenon by enhancing apoptosis.
목차
서론
방법
결과
고찰
REFERENCES
참고문헌 (19)
참고문헌 신청
1. [학술지(정기간행물)] - Pirsch JD / 1997 / A comparison of tacrolimus (FK506) and cyclosporine for immunosuppression after cadaveric renal transplantation / Transplantation 63 : 977 ~ 983
2. [학술지(정기간행물)] - Margreiter R / 2002 / European Tacrolimus vs Ciclosporin Microemulsion Renal Transplantation Study Group. Efficacy and safety of tacrolimus compared with ciclosporin microemulsion in renal transplantation: a randomised multicentre study / Lancet 359 : 741 ~ 746
3. [학술지(정기간행물)] - Mehmet H / 2000 / Caspases find a new place to hide / Nature 403 : 29 ~ 30
4. [학술지(정기간행물)] - Nakagawa T / 2000 / Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-beta / Nature 403 : 98 ~ 103
5. [학술지(정기간행물)] - Morishima N / 2002 / An endoplasmic reticulum stress-specific caspase cascade in apoptosis. Cytochrome c-independent activation of caspase-9 by caspase-12 / J Biol Chem 277 : 34287 ~ 34294
2. [학술지(정기간행물)] - Margreiter R / 2002 / European Tacrolimus vs Ciclosporin Microemulsion Renal Transplantation Study Group. Efficacy and safety of tacrolimus compared with ciclosporin microemulsion in renal transplantation: a randomised multicentre study / Lancet 359 : 741 ~ 746
3. [학술지(정기간행물)] - Mehmet H / 2000 / Caspases find a new place to hide / Nature 403 : 29 ~ 30
4. [학술지(정기간행물)] - Nakagawa T / 2000 / Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-beta / Nature 403 : 98 ~ 103
5. [학술지(정기간행물)] - Morishima N / 2002 / An endoplasmic reticulum stress-specific caspase cascade in apoptosis. Cytochrome c-independent activation of caspase-9 by caspase-12 / J Biol Chem 277 : 34287 ~ 34294
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UCI(KEPA) : I410-ECN-0101-2013-514-002680960