지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2007.9
- 수록면
- 191 - 197 (7page)
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Purpose: FK506 (Tacrolimus) has been widely used as an immunosuppressant. We examined the effects of FK506 on the activation, proliferation and expression of cytotoxic effector molecules of Jurkat human T-lymphocytes.
Methods: We investigated the effects of this compound on cell viability, the production of reactive oxygen species and mitochondrial dysfunction. The cells were cultured in the presence or absence of FK506. Flow cytometric analysis was performed after staining with PI. The viability of the Jurkat cells was decreased by the addition of FK506 in a dose-and time-dependent manners.
Results: FK506-induced cytotoxicity was characterized by G0/G1 phase cell-cycle arrest. FK506 induced cell death was confirmed by the caspase-3 protease activation. In addition, the pharmacologic scavenging study of reactive oxygen species (ROS), including H₂O₂, revealed that cytotoxicity was achieved by the generation of ROS, which might modulate the mitochondrial dysfunction.
Conclusion: These results suggest that FK506 functions in CDK4-cyclin D1 mediated cell-cycle arrest of Jurkat cells via generation of ROS and mitochondrial dysfunction.
Methods: We investigated the effects of this compound on cell viability, the production of reactive oxygen species and mitochondrial dysfunction. The cells were cultured in the presence or absence of FK506. Flow cytometric analysis was performed after staining with PI. The viability of the Jurkat cells was decreased by the addition of FK506 in a dose-and time-dependent manners.
Results: FK506-induced cytotoxicity was characterized by G0/G1 phase cell-cycle arrest. FK506 induced cell death was confirmed by the caspase-3 protease activation. In addition, the pharmacologic scavenging study of reactive oxygen species (ROS), including H₂O₂, revealed that cytotoxicity was achieved by the generation of ROS, which might modulate the mitochondrial dysfunction.
Conclusion: These results suggest that FK506 functions in CDK4-cyclin D1 mediated cell-cycle arrest of Jurkat cells via generation of ROS and mitochondrial dysfunction.
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서론
방법
결과
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REFERENCES
참고문헌 (25)
참고문헌 신청
1. [학술지(정기간행물)] - Meier Kriesche HU / 2003 / Decreased renal function is a strong risk factor for cardiovascular death after renal transplantation / Transplantation 75 : 1291 ~ 1295
2. [학술지(정기간행물)] - Pirsch JD / 1997 / A comparison of tacrolimus (FK506) and cyclosporine for immunosuppression after cadaveric renal transplantation / Transplantation 63 : 977 ~ 983
3. [학술지(정기간행물)] - Liu J / 1991 / Calcineurin is a common target of cyclophilin-cyclosporin A and FKBP-FK506 complexes / Cell 66 : 807 ~ 815
4. [학술지(정기간행물)] - Clipstone NA / 1992 / Identification of calcineurin as a key signalling enzyme in T-lymphocyte activation / Nature 357 : 695 ~ 697
5. [학술지(정기간행물)] - O'Keefe SJ / 1992 / FK-506- and CsA-sensitive activation of the interleukin-2 promoter by calcineurin / Nature 357 : 692 ~ 694
2. [학술지(정기간행물)] - Pirsch JD / 1997 / A comparison of tacrolimus (FK506) and cyclosporine for immunosuppression after cadaveric renal transplantation / Transplantation 63 : 977 ~ 983
3. [학술지(정기간행물)] - Liu J / 1991 / Calcineurin is a common target of cyclophilin-cyclosporin A and FKBP-FK506 complexes / Cell 66 : 807 ~ 815
4. [학술지(정기간행물)] - Clipstone NA / 1992 / Identification of calcineurin as a key signalling enzyme in T-lymphocyte activation / Nature 357 : 695 ~ 697
5. [학술지(정기간행물)] - O'Keefe SJ / 1992 / FK-506- and CsA-sensitive activation of the interleukin-2 promoter by calcineurin / Nature 357 : 692 ~ 694
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UCI(KEPA) : I410-ECN-0101-2013-514-002673069