Phosphatidylcholine regulates NF-κB activation in attenuation of LPS-induced inflammation: evidence from in vitro study

Cheng, Meijuan; Pan, Hongying; Dai, Yining; Zhang, Jiajie; Tong, Yongxi; Huang, Yicheng; Wang, Mingshan; Huang, Haijun

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자료유형: 학술저널

저자정보: Cheng, Meijuan (Department of Infectious Disease, Zhejiang Provincial People's Hospital) Pan, Hongying (Department of Infectious Disease, Zhejiang Provincial People's Hospital) Dai, Yining (Department of Infectious Disease, Zhejiang Provincial People's Hospital) Zhang, Jiajie (Department of Infectious Disease, Zhejiang Provincial People's Hospital) Tong, Yongxi (Department of Infectious Disease, Zhejiang Provincial People's Hospital) Huang, Yicheng (Department of Infectious Disease, Zhejiang Provincial People's Hospital) Wang, Mingshan (Department of Infectious Disease, Zhejiang Provincial People's Hospital) Huang, Haijun (Department of Infectious Disease, Zhejiang Provincial People's Hospital)

저널정보: 한국통합생물학회 Animal cells and systems Animal cells and systems 제22권 제1호

발행연도: 2018.1

수록면: 7 - 14 (8page)

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Phosphatidylcholine (PC) has been demonstrated as anti-inflammatory and antioxidant/pro-oxidant molecules. In this study, we investigated the protective effects of PC on inflammatory bowel disease (IBD) caused by lipopolysaccharide (LPS)-induced injury in intestinal epithelia cells. The IEC-6 cells (intestinal epithelia cells) were stimulated with LPS ($1{\mu}g/mL$) for 24 h with or without PC pretreatment, in the next steps: (1) the level of the inflammatory cytokine tumor necrosis factor (TNF)-${\alpha}$ was measured with ELISA; (2) the nuclear translocation and phosphorylation of NF-${\kappa}B$ was investigated with Western blot, EMSA, immunofluorence assay; (3) the protein phosporylation levels in MAPK signaling pathway were detected with Western blot method. The results showed: (1) compared with the normal group, 10 and $20{\mu}g/mL$ of PC significantly inhibited the production and activation of TNF-${\alpha}$, (P < 0.01); (2) pretreatment with PC inhibited LPS-induced nuclear translocation and phosphorylation of p65 in IEC-6 cells; (3) pretreatment with PC inhibited the protein phosphorylation levels in MAPK signaling pathway. Our findings indicated that PC had the effect to protect IEC-6 cells from LPS-induced injury and this effect was exerted possibly through inhibiting the TNF-${\alpha}$ secretion, down-regulating nuclear translocation and phosphorylation of p65 and inhibiting MAPK signaling pathways.

#Phosphatidylcholine #LPS-induced injury #tTNF- ${alpha}$ secretion and activation #p65 nuclear translocation #MAPKs signal pathway

참고문헌 (25)

참고문헌 신청

Allen A / 2005 / Gastroduodenal mucus bicarbonate barrier: protection against acid and pepsin / Am J Physiol Cell Physiol 288:C1~C19

Atreya I / 2008 / NF-${\kappa}B$ in inflammatory bowel disease / J Intern Med 263:591~596

Barnes PJ / 1997 / Nuclear factor-${\kappa}B$-a pivotal transcription factor in chronic inflammatory diseases / New Eng J Med 336:1066~1071

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