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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 81 - 85 (5page)
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Molecular characterization technology in genetically modified organisms, in addition to how transgenic biotechnologies are developed now require full transparency to assess the risk to living modified and non-modified organisms. Next generation sequencing (NGS) methodology is suggested as an effective means in genome characterization and detection of transgenic insertion locations. In the present study, we applied NGS to insert transgenic loci, specifically the epidermal growth factor (EGF) in genetically modified rice cells. A total of 29.3 Gb (${\sim}72{\times}coverage$) was sequenced with a $2{\times}150bp$ paired end method by Illumina HiSeq2500, which was consecutively mapped to the rice genome and T-vector sequence. The compatible pairs of reads were successfully mapped to 10 loci on the rice chromosome and vector sequences were validated to the insertion location by polymerase chain reaction (PCR) amplification. The EGF transgenic site was confirmed only on chromosome 4 by PCR. Results of this study demonstrated the success of NGS data to characterize the rice genome. Bioinformatics analyses must be developed in association with NGS data to identify highly accurate transgenic sites.
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참고문헌 (13)
참고문헌 신청
Sabalza M. / 2014 / Recombinant plant-derived pharmaceutical proteins : current technical and economic bottlenecks / Biotechnol Lett 36:2367~2379
Schürch C. / 2008 / Potential of plant cells in culture for cosmetic application / Phytochem Rev 7:599~605
Cohen SN. / 1973 / Construction of biologically functional bacterial plasmids in vitro / Proc Natl Acad Sci U S A 70:3240~3244
European Parliament / 2003 / Commission Implementing Regulation (EC) No 1829/2003 of the European Parliament and of the Council of 22 September 2003 on genetically modified food and feed / European Union Legislation. OJ L 268:1~23
European Parliament. / 2003 / REGULATION (EC) No 1830/2003 OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 22 September 2003 concerning the traceability and labelling of genetically modified organisms and the traceability of food and feed products produced from genetically modified organisms and amending Directive 2001/18/EC / European Union Legislation. OJ L 268:24~28
Schürch C. / 2008 / Potential of plant cells in culture for cosmetic application / Phytochem Rev 7:599~605
Cohen SN. / 1973 / Construction of biologically functional bacterial plasmids in vitro / Proc Natl Acad Sci U S A 70:3240~3244
European Parliament / 2003 / Commission Implementing Regulation (EC) No 1829/2003 of the European Parliament and of the Council of 22 September 2003 on genetically modified food and feed / European Union Legislation. OJ L 268:1~23
European Parliament. / 2003 / REGULATION (EC) No 1830/2003 OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 22 September 2003 concerning the traceability and labelling of genetically modified organisms and the traceability of food and feed products produced from genetically modified organisms and amending Directive 2001/18/EC / European Union Legislation. OJ L 268:24~28
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