지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술대회자료
- 저자정보
- 발행연도
- 2021.6
- 수록면
- 205 - 205 (1page)
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Background/Aims
Colorectal cancer (CRC) is the third most common malignancy worldwide. Targeted next-generation sequencing (NGS) technology detects specific mutations that can provide treatment opportunities for colorectal cancer (CRC) patients. We included 145 CRC patients who underwent surgery. We aimed to describe the mutational profile of patients with CRCs using the targeted NGS approach and analyze their potential correlations with clinicopathological factors. In addition, we aimed to assess the biological and clinical significance of low variant allele frequency (VAF) for small variants and compare them with those of The Cancer Genome Atlas (TCGA) and Memorial Sloan Kettering Cancer Center (MSKCC) dataset, a publicly available archive.
Methods
A total of 145 patients with CRC who previously underwent surgical resection in Seoul St. Mary’s hospital between 2016 and 2019 were enrolled. All cases were sporadic clinically, without any familial cancer syndromes. The clinicopathological parameters were retrospectively reviewed from the medical records. DNA and RNA were then extracted using the Recover All Total Nucleic Acid Isolation kit (Ambion, Thermo Fisher Scientific)
Library preparation and sequencing.
Library preparation was accomplished using the Ion Chef System and Oncomine Comprehensive Assay v1 and v3 (Thermo Fisher Scientific, San Francisco, CA, USA). Genomic DNA was then amplified, and targeted gene sequencing was performed using the Ion S5 XL sequencer (Thermo Fisher Scientific, San Francisco, CA, USA). The sequencing data were analyzed with the Ion Torrent Suite ve ... 전체 초록 보기
Colorectal cancer (CRC) is the third most common malignancy worldwide. Targeted next-generation sequencing (NGS) technology detects specific mutations that can provide treatment opportunities for colorectal cancer (CRC) patients. We included 145 CRC patients who underwent surgery. We aimed to describe the mutational profile of patients with CRCs using the targeted NGS approach and analyze their potential correlations with clinicopathological factors. In addition, we aimed to assess the biological and clinical significance of low variant allele frequency (VAF) for small variants and compare them with those of The Cancer Genome Atlas (TCGA) and Memorial Sloan Kettering Cancer Center (MSKCC) dataset, a publicly available archive.
Methods
A total of 145 patients with CRC who previously underwent surgical resection in Seoul St. Mary’s hospital between 2016 and 2019 were enrolled. All cases were sporadic clinically, without any familial cancer syndromes. The clinicopathological parameters were retrospectively reviewed from the medical records. DNA and RNA were then extracted using the Recover All Total Nucleic Acid Isolation kit (Ambion, Thermo Fisher Scientific)
Library preparation and sequencing.
Library preparation was accomplished using the Ion Chef System and Oncomine Comprehensive Assay v1 and v3 (Thermo Fisher Scientific, San Francisco, CA, USA). Genomic DNA was then amplified, and targeted gene sequencing was performed using the Ion S5 XL sequencer (Thermo Fisher Scientific, San Francisco, CA, USA). The sequencing data were analyzed with the Ion Torrent Suite ve ... 전체 초록 보기
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UCI(KEPA) : I410-ECN-0101-2021-514-001919763