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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 459 - 466 (8page)
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초록· 키워드
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Plant-based recombinant protein production systems are powerful tools that can be used instead of microbial and animal cell culture systems. The aim of this study was to establish a stable brazzein production system from carrot cell suspension using bioreactor. Using Agrobacterium-mediated transformation (stress-inducible SWPA2 promoter and brazzein gene), three transgenic lines (TC1, 11, 12) were generated. During the TC12 culture period, cell proliferation increased rapidly up to 15 days, and the maximum cell division rate (log phase) was reached after 6 days of culture. Fresh weight peaked at day 18, 3.8-fold (7.6 g 100 mL?1) higher than on day 1, and gene expression was tenfold higher on day 27 than on day 0. The brazzein-encoding gene was also analyzed after treatment with various stress factors for increase of brazzein gene expression, and abscisic acid (ABA) and hydrogen peroxide ( H2O2) proved most effective. In particular, the gene expression was enhanced 2.5-fold with 220 μM H2O2 and 2.8-fold with 50 μM ABA, compared with controls. Transgenic cells were then transferred to various types of air-lift bioreactor, and the column bioreactor achieved a higher biomass (238.9 g L? 1) than cone and balloon bioreactors. These results demonstrate an efficient protein production system for brazzein using an air-lift bioreactor that may be suitable for the use in the food industry.
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참고문헌 (38)
참고문헌 신청
Assadi-Porter FM / 2000 / Efficient production of recombinant brazzein, a small, heat-stable, sweet-tasting protein of plant origin / Arch Biochem Biophys 376(2):252~258
Berlec A / 2006 / Expression of the sweet-tasting plant protein brazzein in Escherichia coli and Lactococcus lactis : a path toward sweet lactic acid bacteria / Appl Microbiol Biot 73(1):158~165
Chou CP / 2007 / Engineering cell physiology to enhance recombinant protein production in Escherichia coli / Appl Microbiol Biot 76(3):521~532
Deepankumar S / 2019 / Insights on modulators in perception of taste modalities : a review / Nutr Res Rev 32:231~246
Doran PM / 2000 / Foreign protein production in plant tissue cultures / Curr Opinion Biotech 11(2):199~204
Berlec A / 2006 / Expression of the sweet-tasting plant protein brazzein in Escherichia coli and Lactococcus lactis : a path toward sweet lactic acid bacteria / Appl Microbiol Biot 73(1):158~165
Chou CP / 2007 / Engineering cell physiology to enhance recombinant protein production in Escherichia coli / Appl Microbiol Biot 76(3):521~532
Deepankumar S / 2019 / Insights on modulators in perception of taste modalities : a review / Nutr Res Rev 32:231~246
Doran PM / 2000 / Foreign protein production in plant tissue cultures / Curr Opinion Biotech 11(2):199~204
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