지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 63 - 70 (8page)
이용수
초록· 키워드
오류제보하기
Brazzein is the smallest sweet protein and was isolated from the fruit pulp of Pentadiplandra brazzeana Baillon, native to tropical Africa. From ancient times, the indigenous people used this fruit in their diet to add sweetness to their daily food. Brazzein is 500 to 2000 times sweeter than sucrose on a weight basis and 9500 times sweeter on a molar basis. This unique property has led to increasing interest in this protein. However, it is expensive and difficult to produce brazzein other than in its native growing conditions which limits its availability for use as a food additive. In this study, we report high production yields of, brazzein protein in transgenic rice plants. An ORF region encoding brazzein and driven by the 2 x CaMV 35S promoter was introduced into rice genome (Oryza sativa Japonica) via Agrobacterium-mediated transformation. After transformation, 17 regenerated plant lines were obtained and these transgene-containing plants were confirmed by PCR analysis. In addition, the selected plant lines were analyzed by Taqman PCR and results showed that 9 T0 lines were found to have a single copy out of 17 transgenic plants. Moreover, high and genetically stable expression of brazzein was confirmed by western blot analysis. These results demonstrate that recombinant brazzein was efficiently expressed in transgenic rice plants, and that we have developed a new rice variety with a natural sweetener.
목차
등록된 정보가 없습니다.
참고문헌 (41)
참고문헌 신청
Shahina Akter / 2016 / Application of sweet and taste modifying genes for development in plants: current status and prospects / Journal of Plant Biotechnology 43(4):397~404
Assadi-Porter FM / 2000 / Efficient production of recombinant brazzein, a small, heat-stable, sweet-tasting protein of plant origin / Arch Biochem Biophys 376:252~258
Assadi-Porter FM / 2000 / Sweetness Determinant Sites of Brazzein, a Small, Heat-Stable, Sweet-Tasting Protein / Arch Biochem Biophys 376:259~265
Assadi-Porter FM / 2008 / Efficient and rapid protein expression and purification of small high disulfide containing sweet protein brazzein in E. coli / Protein Expr Purif 58:263~268
Berlec A / 2006 / Expression of the sweet-tasting plant protein brazzein in Escherichia coli and Lactococcus lactis: a path toward sweet lactic acid bacteria / Appl microbiol biot 73:158~165
Assadi-Porter FM / 2000 / Efficient production of recombinant brazzein, a small, heat-stable, sweet-tasting protein of plant origin / Arch Biochem Biophys 376:252~258
Assadi-Porter FM / 2000 / Sweetness Determinant Sites of Brazzein, a Small, Heat-Stable, Sweet-Tasting Protein / Arch Biochem Biophys 376:259~265
Assadi-Porter FM / 2008 / Efficient and rapid protein expression and purification of small high disulfide containing sweet protein brazzein in E. coli / Protein Expr Purif 58:263~268
Berlec A / 2006 / Expression of the sweet-tasting plant protein brazzein in Escherichia coli and Lactococcus lactis: a path toward sweet lactic acid bacteria / Appl microbiol biot 73:158~165
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Increased brazzein expression by abiotic stress and bioreactor culture system for the production of sweet protein, brazzein
Plant Biotechnology Reports
2020 .01
-
당근과 벼 캘러스에서 감미단백질 brazzein 생산을 위한 세포배양 시스템 최적화
한국원예학회 학술발표요지
2018 .10
-
Brazzein protein production in transgenic carrot cells using air-lift bioreactor culture
Plant Biotechnology Reports
2022 .04
-
Stable Brazzein Protein Production from Transgenic Carrot Cells by Air-lift Bioreactor Culture
한국원예학회 학술발표요지
2019 .10
-
감미단백질 관련 브라제인, 타우마틴 및 미라쿨린 유전자를 이용한 형질전환 상추육성 및 발현분석
Journal of Plant Biotechnology
2018 .01
-
Application of sweet and taste modifying genes for development in plants: current status and prospects
Journal of Plant Biotechnology
2016 .12
-
감미단백질 모넬린 발현 딸기 형질전환 식물체 개발
Journal of Plant Biotechnology
2015 .01
-
레스베라트롤 합성 GM 벼 검정을 위한 계통특이 마커 개발
한국육종학회지
2016 .01
-
Overexpression of swpa4 peroxidase enhances tolerance to hydrogen peroxide and high salinity-mediated oxidative stress in transgenic sweetpotato plants
Plant Biotechnology Reports
2020 .01
-
무선발 형질전환 Bt 벼 현미의 주요 영양성분 분석
한국육종학회지
2015 .01
-
Agrobacterium을 이용한 제초제 저항성 옥수수 형질전환체 생산
한국육종학회지
2019 .01
-
Concatemer-Associated Transgene Expression Patterns in Transgenic Marine Medaka Oryzias dancena Strains
Fisheries and Aquatic Sciences
2015 .01
-
Overexpression of AtYUCCA6 in soybean crop results in reduced ROS production and increased drought tolerance
Plant Biotechnology Reports
2019 .01
-
Effect of Nitrogen Deficiency on Recombinant Protein Production and Dimerization and Growth in Transgenic Plants
한국원예학회 학술발표요지
2017 .10
-
형질전환 베타카로틴 강화 콩 계통 선발 및 도입유전자 특성 분석
한국육종학회지
2015 .01
-
High-frequency plant regeneration from transgenic rice expressing Arabidopsis thaliana Bax Inhibitor (AtBI-1) tissue cultures
Journal of Plant Biotechnology
2015 .01
-
Development of Protein Biomarkers for the Authentication of Organic Rice
Journal of applied biological chemistry
2015 .01
-
Broad-specificity amino acid racemase, a novel non-antibiotic selectable marker for transgenic plants
Plant Biotechnology Reports
2018 .02
-
Overexpression of a Chromatin Architecture-Controlling ATPG7 has Positive Effect on Yield Components in Transgenic Soybean
Plant Breeding and Biotechnology
2017 .01
-
Overexpression of AtSZF2 from Arabidopsis Showed Enhanced Tolerance to Salt Stress in Soybean
Plant Breeding and Biotechnology
2017 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0