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논문 기본 정보

자료유형
학술저널
저자정보
Lee, Ju-Young (Department of Food Biomaterials, College of Agriculture and Life Sciences, Kyungpook National University) Lim, Jinkyu (Department of Food Biomaterials, College of Agriculture and Life Sciences, Kyungpook National University)
저널정보
한국응용생명화학회 Journal of applied biological chemistry Journal of applied biological chemistry 제58권 제4호
발행연도
2015.1
수록면
355 - 361 (7page)

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The rice protein profiles of Oryza sativa L (Koshihikari) grown under organic and conventional cultivation regimes were compared on 2-D gels to develop diagnostic marker proteins for organic rice. The selected proteins, differentially expressed between organic and conventional rice, were compared with the differentially expressed proteins of another organic and conventional rice pairing, produced at a different location. In the first comparison among conventional, no-chemical, and organic rice grown in the same region, Korea, 13 proteins exhibiting differential expression in organic and conventionally grown plants were selected. Eight of the 13 proteins were down-regulated, and the 5 remaining proteins were up-regulated from conventional to organic rice. The second comparison pairing from Kyungju, revealed 12 differentially expressed proteins, with 8 down-regulated and 4 up-regulated proteins. Ten of the differentially expressed proteins that overlapped between the two comparison sets could not be clustered into any functional group using a functional annotation clustering tool. Further comparisons using another set of conventional and organic rice, belonging to a different variety of Oryza sativa L and produced in Sanchung, revealed 8 differentially expressed proteins, 5 of which were down-regulated and 3 of which were upregulated in the organic rice. Overall, 3 differentially expressed proteins were commonly found in all three organic rice crops. These 3 proteins, along with other overlapping differentially expressed proteins, can provide a good starting point for the development of signature proteins that can be used for the authentication of organic rice with a follow-up studies with more comparison sets.
#2D gel #authentication #conventional #differential expression #endosperm #organic #Oryza sativa L #rice soluble protein

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참고문헌 (22)

참고문헌 신청
Bourn D / 2002 / A comparison of the nutritional value, sensory qualities, and food safety of organically and conventionally produced foods / Critl Rev Food Sci Nutri 42 google schola Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal Biochem 72:248~254 google schola Gammulla CG / 2011 / Differential proteomic response of rice(Oryza sativa)leaves exposed to high-and low-temperature stress / Proteomics 11:2839~2850 google schola Gao B / 2013 / Differentiating organic and conventional sage by chromatographic and mass spectrometry flow injection fingerprints combined with principal component analysis / J Agric Food Chem 61:2957~2963 google schola Guo M / 2004 / Allelic variation of gene expression in maize hybrids / Plant Cell Physiol 16:1707~1716 google schola

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