In Vitro                     Activity of the Novel Tetracyclines, Tigecycline, Eravacycline, and Omadacycline, Against                     Moraxella catarrhalis

Sun Xiang; Zhang Bo; Xu Guangjian; Chen Junwen; Shang Yongpeng; Lin Zhiwei; Yu Zhijian; Zheng Jinxin; Bai Bing

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자료유형: 학술저널

저자정보: Sun Xiang (Department of Infectious Diseases and Shenzhen Key Laboratory for Endogenous Infection Shenzhen Nan) Zhang Bo (Department of Infectious Diseases and Shenzhen Key Laboratory for Endogenous Infection Shenzhen Nan) Xu Guangjian (Department of Infectious Diseases and Shenzhen Key Laboratory for Endogenous Infection Shenzhen Nan) Chen Junwen (Department of Infectious Diseases and Shenzhen Key Laboratory for Endogenous Infection Shenzhen Nan) Shang Yongpeng (Department of Infectious Diseases and Shenzhen Key Laboratory for Endogenous Infection Shenzhen Nan) Lin Zhiwei (Department of Infectious Diseases and Shenzhen Key Laboratory for Endogenous Infection Shenzhen Nan) Yu Zhijian (Department of Infectious Diseases and Shenzhen Key Laboratory for Endogenous Infection Shenzhen Nan) Zheng Jinxin (Department of Infectious Diseases and Shenzhen Key Laboratory for Endogenous Infection Shenzhen Nan) Bai Bing (Department of Infectious Diseases and Shenzhen Key Laboratory for Endogenous Infection Shenzhen Nan)

저널정보: 대한진단검사의학회 Annals of Laboratory Medicine Annals of Laboratory Medicine 제41권 제3호

발행연도: 2021.1

수록면: 293 - 301 (9page)

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Background: Tigecycline, eravacycline, and omadacycline are recently developed tetracyclines. Susceptibility of microbes to these tetracyclines and their molecular mechanisms have not been well elucidated. We investigated the susceptibility of Moraxella catarrhalis to tigecycline, eravacycline, and omadacycline and its resistance mechanisms against these tetracyclines. Methods: A total of 207 non-duplicate M. catarrhalis isolates were collected from different inpatients. The minimum inhibitory concentrations (MICs) of the tetracyclines were determined by broth microdilution. Tigecycline-, eravacycline-, or omadacycline-resistant isolates were induced under in vitro pressure. The tet genes and mutations in the 16S rRNA was detected by PCR and sequencing. Results: Eravacycline had a lower MIC50 (0.06 mg/L) than tigecycline (0.125 mg/L) or omadacycline (0.125 mg/L) against M. catarrhalis isolates. We found that 136 isolates (65.7%) had the tetB gene, and 15 (7.2%) isolates were positive for tetL; however, their presence was not correlated with high tigecycline, eravacycline, or omadacycline (≥1 mg/L) MICs. Compared with the initial MIC after 160 days of induction, the MICs of tigecycline or eravacycline against three M. catarrhalis isolates increased ≥eight-fold, while those of omadacycline against two M. catarrhalis isolates increased 64-fold. Mutations in the 16S rRNA genes (C1036T and/or G460A) were observed in omadacycline-induced resistant isolates, and increased RR (the genes encoding 16SrRNA (four copies, RR1-RR4) copy number of 16S rRNA genes with mutations was associated with increased resistance to omadacycline. Conclusions: Tigecycline, eravacycline, and omadacycline exhibited robust antimicrobial effects against M. catarrhalis. Mutations in the 16S rRNA genes contributed to omadacycline resistance in M. catarrhalis.

#Moraxella catarrhalis #Tigecycline #Eravacycline #Omadacycline #Susceptibility

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