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자료유형
학술저널
저자정보
Jisun Lee (Department of Obstetrics and Gynecology Kyungpook National University Hospital School of Medicine K) Jung Hyeon Yoo (Department of Obstetrics and Gynecology Bundang Jaeseng Hospital Seongnam Korea) Jae Hun Lee (Department of Obstetrics and Gynecology Ajou University School of Medicine Suwon Korea) Hyun Soo Ahn (Department of Urology Ajou University School of Medicine Suwon Korea) Kyung Joo Hwang (Department of Obstetrics and Gynecology Ajou University School of Medicine Suwon Korea) Miran Kim (Department of Obstetrics and Gynecology Ajou University School of Medicine Suwon Korea)
저널정보
영남대학교 의과대학 Journal of Yeungnam Medical Science Yeungnam University Journal of Medicine 제38권 제1호
발행연도
2021.1
수록면
53 - 59 (7page)

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Background: There are no guidelines for the optimal incubation time or temperature to improve pregnancy outcomes in testicular sperm extraction-intracytoplasmic sperm injection (TESE-ICSI) cycles. We aimed to evaluate whether a 24-hour in vitro culture of testicular spermatozoa affects pregnancy outcomes in TESE-ICSI cycles. Methods: This was a retrospective study of 83 TESE-ICSI cycles using testicular spermatozoa in 46 couples with male partners suffering from nonobstructive or obstructive azoospermia. Sperm retrieval was performed either on the oocyte retrieval (OR) day (65 cycles in 33 couples; group A) or on the day before OR (18 cycles in 13 couples; group B) followed by in vitro culture for 24 hours. The clinical characteristics and pregnancy outcomes, including the number of retrieved oocytes, fertilization rates, embryo transfer rates, implantation and clinical pregnancy rates, were compared between the two groups. Results: There were no differences in terms of clinical characteristics except for the levels of luteinizing hormone (LH) in males. Group B had higher LH levels than group A (4.56±1.24 IU/L vs. 3.67±1.07 IU/L, p=0.017). Group B showed higher fertilization rate (72.4±32.1% vs. 59.2±21.7%, p=0.045), implantation rate (35.0±34.1% vs. 14.0±21.5%, p=010), pregnancy rate per cycle (80% vs. 39%, p=0.033), and clinical pregnancy rate per cycle (80% vs. 37.5%, p=0.024) than those of group A. Conclusion: Testicular sperm retrieval performed on the day before OR followed by in vitro culture can potentially improve pregnancy outcomes.

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