Long Noncoding RNA PVT1 Promotes Stemness and Temozolomide Resistance through miR-365/ELF4/SOX2 Axis in Glioma

Gong Rui; Li Zhi-Qiang; Fu Kai; Ma Chao; Wang Wei; Chen Jin-Cao

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자료유형: 학술저널

저자정보: Gong Rui (Departments of Neurosurgery Zhongnan Hospital of Wuhan University Wuchang 430071 Hubei P.R. China) Li Zhi-Qiang (Departments of Neurosurgery Zhongnan Hospital of Wuhan University Wuchang 430071 Hubei P.R. China) Fu Kai (Departments of Neurosurgery Zhongnan Hospital of Wuhan University Wuchang 430071 Hubei P.R. China) Ma Chao (Departments of Neurosurgery Zhongnan Hospital of Wuhan University Wuchang 430071 Hubei P.R. China) Wang Wei (Departments of Neurosurgery Zhongnan Hospital of Wuhan University Wuchang 430071 Hubei P.R. China) Chen Jin-Cao (Departments of Neurosurgery Zhongnan Hospital of Wuhan University Wuchang 430071 Hubei P.R. China)

저널정보: 한국뇌신경과학회 Experimental Neurobiology Experimental Neurobiology Vol.30 No.3

발행연도: 2021.1

수록면: 244 - 255 (12page)

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Long non-coding RNA (lncRNA) are a class of non-coding RNAs demonstrated to play pivotal roles in regulating tumor progression. Therefore, deciphering the regulatory role of lncRNA in the development of glioma may offer a promising therapeutic target for treatment of glioma. We per formed RT-qPCR analysis on the expression of lncRNA plasmacytoma variant translocation 1 (PVT1) and miR-365 in glioma tissues and cell lines. Cell proliferation and viability was assessed with CCK8 assay. Cell migration was assessed by wound healing assay. Transwell assay was used to as sess cell invasion capacity. Expression of CD133+ cells was detected by flow cytometry. Western blot assay was used to detection the expression of ELF4 and stemness-related protein SOX2, Oct4 and Nanog. Bioinformatics and dual-luciferase assay were used to predict and validate the interac tion between PVT1 and miR-365. Elevated PVT1 expression was observed in glioma tissues and cells. Knockdown of PVT1 and overexpression of miR-365 inhibited proliferation, migration, invasion and promoted stemness and Temozolomide (TMZ) resistance of glioma cells. PVT1 regulated ELF4 expression by competitively binds to miR-365. PVT1 regulated the stemness and sensitivity of TMZ of glioma cells through miR-365/ELF4/ SOX2 axis. This study identified that PVT1 promoted glioma stemness through miR-365/ELF4/SOX2 axis.

#lncRNA PVT1 #miR-365 #ELF4 #SOX2 #Glioma

참고문헌 (38)

참고문헌 신청

Davis ME / 2016 / Glioblastoma: overview of disease and treatment / Clin J Oncol Nurs 20(5 Suppl):S2~S8

Cheng Z / 2017 / Long non-coding RNA XIST promotes glioma tumorigenicity and angiogenesis by acting as a molecular sponge of miR-429 / J Cancer 8:4106~4116

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Jackson M / 2015 / Glioblastoma stemlike cells : at the root of tumor recurrence and a therapeutic target / Carcinogenesis 36:177~185

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