The potential efficacy of the E2-subunit vaccine to protect pigs against different genotypes of classical swine fever virus circulating in Vietnam

Ha Thi Thanh Tran; Duc Anh Truong; Viet Duc Ly; Hao Thi Vu; Tuan Van Hoang; Chinh Thi Nguyen; Nhu Thi Chu; Vinh The Nguyen; Duyen Thuy Nguyen; Kohtaroh Miyazawa; Takehiro Kokuho; Hoang Vu Dang

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저자정보: Ha Thi Thanh Tran (Department of Biochemistry and Immunology National Institute of Veterinary Research Vietnam) Duc Anh Truong (National Institute of Veterinary Research (NIVR)) Viet Duc Ly (National Institute of Veterinary Research (NIVR)) Hao Thi Vu (National Institute of Veterinary Research (NIVR)) Tuan Van Hoang (National Institute of Veterinary Research (NIVR)) Chinh Thi Nguyen (National Institute of Veterinary Research (NIVR)) Nhu Thi Chu (National Institute of Veterinary Research (NIVR)) Vinh The Nguyen (National Institute of Veterinary Research (NIVR)) Duyen Thuy Nguyen (National Institute of Veterinary Research (NIVR)) Kohtaroh Miyazawa (The National Agriculture and Food Research Organization) Takehiro Kokuho (The National Agriculture and Food Research Organization) Hoang Vu Dang (National Institute of Veterinary Research (NIVR))

저널정보: 대한백신학회 Clinical and Experimental Vaccine Research Clinical and Experimental Vaccine Research Vol.9 No.1

발행연도: 2020.1

수록면: 26 - 39 (14page)

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Purpose: To date, many kinds of classical swine fever (CSF) vaccines have been developed to protect against this disease. However, the efficacy of these vaccines to protect the pig against field CSF strains needs to be considered, based on circulating strains of classical swine fever virus (CSFV). Materials and Methods: Recombinant E2-CSFV protein produced by baculovirus/insect cell system was analyzed by western blots and immunoperoxidase monolayer assay. The effect of CSFV-E2 subunit vaccines was evaluated in experimental pigs with three genotypes of CSFV challenge. Anti-E2 specific and neutralizing antibodies in experimental pigs were analyzed by blocking enzyme-linked immunosorbent assay and neutralization peroxidize-linked assay. Results: The data showed that CSFV VN91-E2 subunit vaccine provided clinical protection in pigs against three different genotypes of CSFV without noticeable clinical signs, symptoms, and mortality. In addition, no CSFV was isolated from the spleen of the vaccinated pigs. However, the unvaccinated pigs exhibited high clinical scores and the successful virus isolation from spleen. These results showed that the E2-specific and neutralizing antibodies induced by VN91-E2 antigen appeared at day 24 after first boost and a significant increase was observed at day 28 (p<0.01). This response reached a peak at day 35 and continued until day 63 when compared to controls. Importantly, VN91-E2 induced E2-specific and neutralizing antibodies protected experimental pigs against high virulence of CSFVs circulating in Vietnam, including genotype 1.1, 2.1, and 2.2. Conclusion: These findings also suggested that CSFV VN91-E2 subunit vaccine could be a promising vaccine candidate for the control and prevention of CSFV in Vietnam.

#Classical swine fever #Genotype #Vaccine #Vietnam

참고문헌 (35)

참고문헌 신청

Moennig V / 2003 / Clinical signs and epidemiology of classical swine fever : a review of new knowledge / Vet J 165:11~20

Paton DJ / 2000 / Genetic typing of classical swine fever virus / Vet Microbiol 73:137~157

Gomez-Villamandos JC / 2003 / African swine fever and classical swine fever : a review of the pathogenesis / Dtsch Tierarztl Wochenschr 110:165~169

Paton DJ / 2003 / Classical swine fever : an update / Res Vet Sci 75:169~178

Blome S / 2017 / Classical swine fever-an updated review / Viruses 9

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Ha Thi Thanh Tran

소속기관 Department of Biochemistry and Immunology National Institute of Veterinary Research Vietnam