Identification and expression patterns of 20-hydroxyecdysoneresponsive genes from Procambarus clarkii

Bao‑Jian Zhu; Lin Tang; Ying‑Ying Yu; Dao‑Jun Wang; Chao‑Liang Liu

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자료유형: 학술저널

저자정보: Bao‑Jian Zhu (Anhui Agricultural University) Lin Tang (Anhui Agricultural University) Ying‑Ying Yu (Anhui Agricultural University) Dao‑Jun Wang (Anhui Agricultural University) Chao‑Liang Liu (Anhui Agricultural University)

저널정보: 한국유전학회 Genes & Genomics Genes & Genomics Vol.39 No.6

발행연도: 2017.1

수록면: 601 - 609 (9page)

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Ecdysteroids play an important role in the regulation of molting process in crustaceans. The suppression subtractive hybridization (SSH) technique was employed to search for 20-hydroxyecdysone-responsive genes in the hepatopancreas of the crayfish Procambarus clarkii. Ninety-three putative expressed sequence tags were identified by SSH, including three immune response-related genes, two cell cycle and apoptosis genes, four respiration and energy metabolism genes, four transport-related genes, six metabolism-related genes, two stress response genes, and eight transcription and translation regulation genes. The expression levels of the examined genes were regulated by 20-hydroxyecdysone and varied in the hepatopancreas during the molting stages; data were confirmed by real-time PCR. RNAi of the ecdysteroid receptor had significant effects on the expression levels of 20-hydroxyecdysone- responsive genes. These differentially expressed genes identified by SSH will provide insight into the ecdysteroid signaling pathway in P. clarkii.

참고문헌 (53)

참고문헌 신청

Aragon S / 2002 / Molting cycle-dependent expression of CYP4C15, a cytochrome P450 enzyme putatively involved in ecdysteroidogenesis in the crayfish, Orconectes limosus / Insect Biochem Mol Biol 32:153~159

Asazuma H / 2007 / Molecular cloning and expression analysis of ecdysone receptor and retinoid X receptor from the kuruma prawn, Marsupenaeus japonicus / Comp Biochem Physiol 148:139~150

Bacqué-Cazenave J / 2013 / Control of motor activity in crayfish by the steroid hormone 20-hydroxyecdysone via motoneuron excitability and sensory-motor integration / J Exp Biol 216:1808~1818

Bagamasbad P / 2011 / Mechanisms and significance of nuclear receptor auto-and cross-regulation / Gen Comp Endocr 170:3~17

Barrett AJ / 2001 / Evolutionary lines of cysteine peptidases / J Biol Chem 382:727~733

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