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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 405 - 416 (12page)
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Background: To develop surrogate insulin-producing cells for diabetes therapy, adult stem cells have been identified in various tissues and studied for their conversion into β-cells. Pancreatic progenitor cells are derived from the endodermal epithelium and formed in a manner similar to gut progenitor cells. Here, we generated insulin-producing cells from the intestinal epithelial cells that induced many of the specific pancreatic transcription factors using adenoviral vectors carrying three genes: PMB (pancreatic and duodenal homeobox 1 [Pdx1], V-maf musculoaponeurotic fibrosarcoma oncogene homolog A [MafA], and BETA2/NeuroD). Methods: By direct injection into the intestine through the cranial mesenteric artery, adenoviruses (Ad) were successfully delivered to the entire intestine. After virus injection, we could confirm that the small intestine of the mouse was appropriately infected with the Ad-Pdx1 and triple Ad-PMB. Results: Four weeks after the injection, insulin mRNA was expressed in the small intestine, and the insulin gene expression was induced in Ad-Pdx1 and Ad-PMB compared to control Ad-green fluorescent protein. In addition, the conversion of intestinal cells into insulin-expressing cells was detected in parts of the crypts and villi located in the small intestine. Conclusion: These data indicated that PMB facilitate the differentiation of mouse intestinal cells into insulin-expressing cells. In conclusion, the small intestine is an accessible and abundant source of surrogate insulin-producing cells.
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참고문헌 (39)
참고문헌 신청
Shapiro AM / 2000 / Islet transplantation in seven patients with type 1 diabetes mellitus using a glucocorticoid-free immunosuppressive regimen / N Engl J Med 343:230~238
Hua H / 2013 / iPSC-derived β cells model diabetes due to glucokinase deficiency / J Clin Invest 123:3146~3153
Bonner-Weir S / 2005 / New sources of pancreatic beta-cells / Nat Biotechnol 23:857~861
Song KH / 2004 / In vitro transdifferentiation of adult pancreatic acinar cells into insulin-expressing cells / Biochem Biophys Res Commun 316:1094~1100
Thorel F / 2010 / Conversion of adult pancreatic alpha-cells to beta-cells after extreme beta-cell loss / Nature 464:1149~1154
Hua H / 2013 / iPSC-derived β cells model diabetes due to glucokinase deficiency / J Clin Invest 123:3146~3153
Bonner-Weir S / 2005 / New sources of pancreatic beta-cells / Nat Biotechnol 23:857~861
Song KH / 2004 / In vitro transdifferentiation of adult pancreatic acinar cells into insulin-expressing cells / Biochem Biophys Res Commun 316:1094~1100
Thorel F / 2010 / Conversion of adult pancreatic alpha-cells to beta-cells after extreme beta-cell loss / Nature 464:1149~1154
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