Ginseng-derived nanoparticles induce skin cell proliferation and promote wound healing

Song Yang; Shuyan Lu; Limei Ren; Shuai Bian; Daqing Zhao; Meichen Liu; Jiawen Wang

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자료유형: 학술저널

저자정보: Song Yang (Changchun University of Chinese Medicine) Shuyan Lu (Changchun University of Chinese Medicine) Limei Ren (Changchun University of Chinese Medicine) Shuai Bian (Changchun University of Chinese Medicine) Daqing Zhao (Changchun University of Chinese Medicine) Meichen Liu (Changchun University of Chinese Medicine) Jiawen Wang (Changchun University of Chinese Medicine)

저널정보: 고려인삼학회 Journal of Ginseng Research Journal of Ginseng Research Vol.47 No.1

발행연도: 2023.1

수록면: 133 - 143 (11page)

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Background: Past studies suggested that ginseng extracts and ginseng-derived molecules exerted significant regulatory effects on skin. However, no reports have described the effects of ginseng-derived nanoparticles (GDNPs) on skin cell proliferation and wound healing. In this study, we investigated whether GDNPs regulate the proliferation of skin cells and promote wound healing in a mouse model.
Methods: GDNPs were separated and purified via differential centrifugation and sucrose/D2O gradient ultracentrifugation. GDNP uptake, cell proliferation and cell cycle progression were measured by confocal microscopy, CCK-8 assay and flow cytometry, respectively. Cell migration and angiogenic effects were assessed by the wound scratch assay and tube formation assay, respectively. ELISA was used to detect extracellular matrix secretion. The relevant signaling pathway was confirmed by western blotting. The effects of GDNPs on skin wound healing were assessed by wound observation, HE staining, and western blotting.
Results: GDNPs possessed the essential features of exosomes, and they were accumulated by skin cells. Treatment with GDNPs notably enhanced the proliferation of HaCaT, BJ and HUVECs. GDNPs also enhanced the migration in HaCaT cells and HUVECs and angiogenesis in HUVECs. GDNPs increased the secretion of MMP-1, fibronectin-1, elastin-1, and COL1A1 in all three cell lines. GDNPs regulated cell proliferation through the ERK and AKT/ mTOR pathways. Furthermore, GDNPs facilitated skin wound healing and decreased inflammation in a mouse skin wound model.
Conclusion: GDNPs can promote skin wound healing through the ERK and AKT/mTOR pathways. GDNPs thus represent an alternative treatment for chronic skin wounds.

#cell cycle #cell proliferation #ginseng-derived nanoparticles #migration #wound healing

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