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논문 기본 정보

자료유형
학술저널
저자정보
Yosboonruang Atchariya (Division of Microbiology School of Medical Sciences University of Phayao Phayao 56000 Thailand) Ontawong Atcharaporn (Unit of Excellence in Research and Product Development of Coffee Division of Physiology School of M) Thapmamang Jadsada (Division of Microbiology School of Medical Sciences University of Phayao Phayao 56000 Thailand) Duangjai Acharaporn (Unit of Excellence in Research and Product Development of Coffee Division of Physiology School of M)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제32권 제8호
발행연도
2022.8
수록면
1,003 - 1,010 (8page)
DOI
10.4014/jmb.2204.04003

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The purpose of this study was to examine the phytochemical compounds and antibacterial activity of Coffea robusta leaf extract (RLE). The results indicated that chlorogenic acid (CGA) is a major component of RLE. The minimum inhibitory concentrations (MICs) of RLE against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Salmonella Typhimurium were 6.25, 12.5, 12.5, and 12.5 mg/ml, respectively. RLE effectively damages the bacterial cell membrane integrity, as indicated by the high amounts of proteins and nucleic acids released from the bacteria, and disrupts bacterial cell membrane potential and permeability, as revealed via fluorescence analysis. Cytotoxicity testing showed that RLE is slightly toxic toward HepG2 cells at high concentration but exhibited no toxicity toward Caco2 cells. The results from the present study suggest that RLE has excellent potential applicability as an antimicrobial in the food industry.

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