지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.9
- 수록면
- 621 - 631 (11page)
- DOI
- 10.14348/molcells.2017.0108
이용수
초록· 키워드
오류제보하기
Vaccinia-related kinase 1 (VRK1) and VRK3 are members of the VRK family of serine/threonine kinases and are principally localized in the nucleus. Despite the crucial roles of VRK1/ VRK3 in physiology and disease, the molecular and functional interactions of VRK1/VRK3 are poorly understood. Here, we identified over 200 unreported VRK1/VRK3-interacting candidate proteins by affinity purification and LC-MS/MS. The networks of VRK1 and VRK3 interactomes were found to be associated with important biological processes such as the cell cycle, DNA repair, chromatin assembly, and RNA processing. Interactions of interacting proteins with VRK1/VRK3 were confirmed by biochemical assays. We also found that phosphorylations of XRCC5 were regulated by both VRK1/VRK3, and that of CCNB1 was regulated by VRK3. In liver cancer cells and tissues, VRK1/VRK3 were highly upregulated and its depletion affected cell cycle progression in the different phases. VRK3 seemed to affect S phase progression and G2 or M phase entry and exit, whereas VRK1 affects G1/S transition in the liver cancer, which could be explained by different interacting candidate proteins. Thus, this study not only provides a resource for investigating the unidentified func-tions of VRK1/ VRK3, but also an insight into the regulatory roles of VRK1/VRK3 in biological processes.
목차
등록된 정보가 없습니다.
참고문헌 (51)
참고문헌 신청
Choi, D. S. / 2011 / Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa / Proteomics 11:3424~3429
Choi, Y. H. / 2012 / HnRNP A1phosphorylated by VRK1 stimulates telomerase and its binding to telomeric DNA sequence / Nucleic Acids Res 40:8499~8518
Douglas, P. / 2005 / DNA-PK-dependent phosphorylation of Ku70/80 is not required for non-homologous end joining / DNA Repair 4:1006~1018
Gao, Y. / 2005 / Spindlin1, a novel nuclear protein with a role in the transformation of NIH3T3 cells / Biochem. Biophys. Res. Commun 335:343~350
Gavet, O. / 2010 / Progressive activation of CyclinB1-Cdk1 coordinates entry to mitosis / Dev. Cell 18:533~543
Choi, Y. H. / 2012 / HnRNP A1phosphorylated by VRK1 stimulates telomerase and its binding to telomeric DNA sequence / Nucleic Acids Res 40:8499~8518
Douglas, P. / 2005 / DNA-PK-dependent phosphorylation of Ku70/80 is not required for non-homologous end joining / DNA Repair 4:1006~1018
Gao, Y. / 2005 / Spindlin1, a novel nuclear protein with a role in the transformation of NIH3T3 cells / Biochem. Biophys. Res. Commun 335:343~350
Gavet, O. / 2010 / Progressive activation of CyclinB1-Cdk1 coordinates entry to mitosis / Dev. Cell 18:533~543
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