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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.6
- 수록면
- 532 - 544 (13page)
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초록· 키워드
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Gastrointestinal stromal tumours (GIST) are the most common mesenchymal tumors of the gastrointestinal (GI) tract. In order to investigate a new treatment fot GIST, we hypothesized the effect of miR-374b targeting PTEN gene-mediated PI3K/Akt signal transduction pathway on proliferation and apoptosis of human gastrointestinal stromal tumor (GIST) cells. We obtained GIST tissues and adjacent normal tissues from 143 patients with GIST to measure the levels of miR-374b, PTEN, PI3K, Akt, caspase9, Bax, MMP2, MMP9, ki67, PCNA, P53 and cyclinD1. Finally, cell viability, cell cycle and apoptosis were detected. According to the KFGG analysis of DEGs, PTEN was involved in a variety of signaling pathways and miRs were associated with cancer development. The results showed that MiR-374b was highly expressed, while PTEN was downregulated in the GIST tissues. The levels of miR-374b, PI3K, AKT and PTEN were related to tumor diameter and pathological stage. Additionally, miR-374b increased the mRNA and protein levels of PI3K, Akt, MMP2, MMP9, P53 and cyclinD1, suggesting that miR-374b activates PI3K/Akt signaling pathway in GIST-T1 cells. Moreover, MiR-374b promoted cell viability, migration, invasion, and cell cycle entry, and inhibited apoptosis in GIST cells. Taken together, the results indicated that miR-374b pro-motes viability and inhibits apoptosis of human GIST cells by targeting PTEN gene through the PI3K/Akt signaling pathway. Thus, this study provides a new potential target for GIST treatment.
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참고문헌 (35)
참고문헌 신청
Cao, C.L. / 2016 / miRNA-21 sensitizes gastrointestinal stromal tumors (GISTs) cells to Imatinib via targeting B-cell lymphoma 2 (Bcl-2) / Eur. Rev. Med. Pharmacol. Sci. 20:3574~3581
Chen, L. / 2016 / MiR-126 inhibits vascular endothelial cell apoptosis through targeting PI3K/Akt signaling / Ann. Hematol. 95:365~374
Choi, H.J. / 2010 / MicroRNA expression profile of gastrointestinal stromal tumors is distinguished by 14q loss and anatomic site / Int. J. Cancer 126:1640~1650
Dong, P. / 2014 / The impact of microRNA-mediated PI3K/AKT signaling on epithelial-mesenchymal transition and cancer stemness in endometrial cancer / J. Transl. Med. 12:231
Fresno Vara, J.A. / 2004 / PI3K/Akt signalling pathway and cancer / Cancer Treat. Rev. 30:193~204
Chen, L. / 2016 / MiR-126 inhibits vascular endothelial cell apoptosis through targeting PI3K/Akt signaling / Ann. Hematol. 95:365~374
Choi, H.J. / 2010 / MicroRNA expression profile of gastrointestinal stromal tumors is distinguished by 14q loss and anatomic site / Int. J. Cancer 126:1640~1650
Dong, P. / 2014 / The impact of microRNA-mediated PI3K/AKT signaling on epithelial-mesenchymal transition and cancer stemness in endometrial cancer / J. Transl. Med. 12:231
Fresno Vara, J.A. / 2004 / PI3K/Akt signalling pathway and cancer / Cancer Treat. Rev. 30:193~204
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