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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.10
- 수록면
- 917 - 922 (6page)
- DOI
- 10.14348/molcells.2018.0209
이용수
초록· 키워드
오류제보하기
The CRISPR-Cas system is a well-established RNA-guided DNA editing technique widely used to modify genomic DNA sequences. I used the CRISPR-Cas9 system to change the second and third nucleotides of the triplet TCT of human TNSFSF9 in HepG2 cells to TAG to create an amber stop codon. The TCT triplet is the codon for Ser at the 172nd position of TNSFSF9. The two substituted nucleotides, AG, were confirmed by DNA sequencing of the PCR product followed by PCR amplification of the genomic TNFSF9 gene. Interestingly, sequencing of the cDNA of transcripts of the edited TNFSF9 gene revealed that the TAG had been re-edited to the wild type triplet TCT, and 1 or 2 bases just before the triplet had been deleted. These observations indicate that CRISPR-Cas9-mediated editing of bases in target genomic DNA can be followed by spontaneous re-editing (correcting) of the bases during transcription.
목차
등록된 정보가 없습니다.
참고문헌 (32)
참고문헌 신청
Baltimore, D. / 2015 / Biotechnology. A prudent path forward for genomic engineering and germline gene modification / Science 348:36~38
Bibikova, M. / 2002 / Targeted chromosomal cleavage and mutagenesis in drosophila using zincfinger nucleases / Genetics 161:1169~1175
Bibikova, M. / 2003 / Enhancing gene targeting with designed zinc finger nucleases / Science 300:764
Boch, J. / 2009 / Breaking the code of DNA binding specificity of TAL-type III effectors / Science 326:1509~1512
Choulika, A. / 1995 / Induction of homologous recombination in mammalian chromosomes by using the I-SceI system of Saccharomyces cerevisiae / Mol. Cell. Biol. 15:1968~1973
Bibikova, M. / 2002 / Targeted chromosomal cleavage and mutagenesis in drosophila using zincfinger nucleases / Genetics 161:1169~1175
Bibikova, M. / 2003 / Enhancing gene targeting with designed zinc finger nucleases / Science 300:764
Boch, J. / 2009 / Breaking the code of DNA binding specificity of TAL-type III effectors / Science 326:1509~1512
Choulika, A. / 1995 / Induction of homologous recombination in mammalian chromosomes by using the I-SceI system of Saccharomyces cerevisiae / Mol. Cell. Biol. 15:1968~1973
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