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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.12
- 수록면
- 1,371 - 1,379 (9page)
- DOI
- 10.1007/s13258-020-01019-2
이용수
초록· 키워드
오류제보하기
Background Chronic obstructive pulmonary disease (COPD) is a chronic lung disease and the third leading cause of death in the world. Dexmedetomidine has been reported to efectively inhibit histamine-induced bronchoconstriction. However, the molecular mechanism of dexmedetomidine in COPD has not been found.
Objective To explore the role and mechanism of dexmedetomidine in COPD, and to provide theoretical basis for clinical treatment of COPD.
Methods The expression of miR-146a was regulated by mimics or inhibitor and the relative expression of apoptotic proteins p53, Bax and Bcl-2 in human bronchial epithelial 16HBE cells was determined by real-time PCR and Western blot. Dexmedetomidine was treated for 16HBE cells and alveolar epithelial type II cells (AEC2), the cell apoptosis was detected by TUNEL and Hoechst33342 staining. A COPD rat model was established by smoking to test the efects of dexmedetomidine on the progression of COPD. The levels of IL-6, IL-1β and TNF-α in serum were measured by ELISA and the protein concentration of bronchoalveolar lavage fuid (BALF) was also detected in dexmedetomidine treated COPD rat model.
Results miR-146a promoted 16HBE cell apoptosis and reduced cell proliferation. Additionally, dexmedetomidine was showed to reduce the 16HBEL cell apoptosis through reducing the expression of miR-146a. Moreover, dexmedetomidine regulated cell apoptosis and cell apoptosis through miR-146a in AEC2 cells. More importantly, dexmedetomidine attenuated the morphology and pathology of COPD rat model.
Conclusion Dexmedetomidine reduced 16HBE cells and AEC2 cell apoptosis and attenuated COPD by down-regulating miR-146a.
목차
등록된 정보가 없습니다.
참고문헌 (26)
참고문헌 신청
Bartel DP / 2009 / MicroRNAs : target recognition and regulatory functions / Cell 136:215~233
Demedts IK / 2006 / Role of apoptosis in the pathogenesis of COPD and pulmonary emphysema / Respir Res 7:53
Ezzie ME / 2012 / Gene expression networks in COPD : microRNA and mRNA regulation / Thorax 67:122~131
Frerking I / 2001 / Pulmonary surfactant : functions, abnormalities and therapeutic options / Intensive Care Med 27:1699~1717
Garbacki N / 2011 / MicroRNAs profling in murine models of acute and chronic asthma : a relationship with mRNAs targets / PLoS ONE 6:e16509
Demedts IK / 2006 / Role of apoptosis in the pathogenesis of COPD and pulmonary emphysema / Respir Res 7:53
Ezzie ME / 2012 / Gene expression networks in COPD : microRNA and mRNA regulation / Thorax 67:122~131
Frerking I / 2001 / Pulmonary surfactant : functions, abnormalities and therapeutic options / Intensive Care Med 27:1699~1717
Garbacki N / 2011 / MicroRNAs profling in murine models of acute and chronic asthma : a relationship with mRNAs targets / PLoS ONE 6:e16509
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