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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한약리학회 The Korean Journal of Physiology & Pharmacology The Korean Journal of Physiology & Pharmacology 제21권 제5호
- 발행연도
- 2017.9
- 수록면
- 531 - 546 (16page)
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Activation of Toll-like receptor-4 (TLR-4) in articular chondrocytes increases the catabolic compartment and leads to matrix degradation during the development of osteoarthritis. In this study, we determined the proteomic and genomic alterations in human chondrocytes during lipopolysaccharide (LPS)- induced inflammation to elucidate the underlying mechanisms and consequences of TLR-4 activation. Human chondrocytes were cultured with LPS for 12, 24, and 36 h to induce TLR-4 activation. The TLR-4-induced inflammatory response was confirmed by real-time PCR analysis of increased interleukin-1 beta (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor alpha (TNF-α) expression levels.
In TLR-4-activated chondrocytes, proteomic changes were determined by twodimensional electrophoresis and matrix-assisted laser desorption/ionization-mass spectroscopy analysis, and genomic changes were determined by microarray and gene ontology analyses. Proteomics analysis identified 26 proteins with significantly altered expression levels; these proteins were related to the cytoskeleton and oxidative stress responses. Gene ontology analysis indicated that LPS treatment altered specific functional pathways including ‘chemotaxis’, ‘hematopoietic organ development’, ‘positive regulation of cell proliferation’, and ‘regulation of cytokine biosynthetic process’. Nine of the 26 identified proteins displayed the same increased expression patterns in both proteomics and genomics analyses. Western blot analysis confirmed the LPS-induced increases in expression levels of lamin A/C and annexins 4/5/6. In conclusion, this study identified the time-dependent genomic, proteomic, and functional pathway alterations that occur in chondrocytes during LPS-induced TLR-4 activation. These results provide valuable new insights into the underlying mechanisms that control the development and progression of osteoarthritis.
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참고문헌 (94)
참고문헌 신청
Sophia Fox AJ / 2009 / The basic science of articular cartilage : structure, composition, and function / Sports Health 1:461~468
Campo GM / 2010 / Molecular size hyaluronan differently modulates toll-like receptor-4 in LPS-induced inflammation in mouse chondrocytes / Biochimie 92:204~215
Sanchez-Adams J / 2014 / The mechanobiology of articular cartilage : bearing the burden of osteoarthritis / Curr Rheumatol Rep 16:451
Gaston JS / 2008 / Cytokines in arthritis--the 'big numbers' move centre stage / Rheumatology(Oxford) 47:8~12
Finckh A / 2008 / At the horizon of innovative therapy in rheumatology : new biologic agents / Curr Opin Rheumatol 20:269~275
Campo GM / 2010 / Molecular size hyaluronan differently modulates toll-like receptor-4 in LPS-induced inflammation in mouse chondrocytes / Biochimie 92:204~215
Sanchez-Adams J / 2014 / The mechanobiology of articular cartilage : bearing the burden of osteoarthritis / Curr Rheumatol Rep 16:451
Gaston JS / 2008 / Cytokines in arthritis--the 'big numbers' move centre stage / Rheumatology(Oxford) 47:8~12
Finckh A / 2008 / At the horizon of innovative therapy in rheumatology : new biologic agents / Curr Opin Rheumatol 20:269~275
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