지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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초록· 키워드
오류제보하기
Cystathionine gamma synthase from Corynebacterium glutamicum (CgMetB) is a key enzyme for the production ofL-methionine and it condenses O-acetyl-L-homoserine (OAHS) and cysteine to produce cystathionine. MetB is also anattractive target for the development of antimicrobial compounds because it catalyzes the first reaction of the L-methioninebiosynthetic pathway. The CgMetB was overexpressed and purified to homogeneity by affinity and size-exclusionchromatography. The CgMetB protein was crystallized using hanging-drop vapor-diffusion method in the presence of13% polyethylene glycol 3350 and 0.1 M Magnesium formate dihydrate at 295 K. X-ray diffraction data were collected to amaximum resolution of 1.5 A. The crystal belonged to space group F222, with unit cell parameters a = 58.57 A, b = 149.85 A,c = 161.86, α = β = γ = 90.0°. With one molecules per asymmetric unit, the crystal volume per unit protein mass was 2.13 A3Da-1, which correspond to a solvent content of approximately 42.27%.
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등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Becker, J / 2011 / From zero to hero-design-based systems metabolic engineering of Corynebacterium glutamicum for l-lysine production / Metab Eng 13:159~168
Berman, H. M / 2000 / The Protein Data Bank / Nucleic Acids Res 28:235~242
Chibata, I / 1957 / Studies on Amino Acids: V. Studies on the Enzymatic Resolution (IV). Enzymatic Resolution of DL-Methionine (2) VI. Studies on the Enzymatic Resolution (V). Enzymatic Resolution of DL-Lysine (2) VII. Studies on the Enzymatic Resolution (VI). A Survey of the Acylase in Molds VIII. Studies on the Enzymatic Resolution (VII). Specificity of Mold Acylase / Bull Agric Chem Soc Japan 21:291~307
Clausen, T / 1998 / Crystal structure of Escherichia coli cystathionine g-synthase at 1. 5 Å / EMBO J 17:6827~6838
Clifton, M. C / 2011 / Structue of the cystathionine g-synthase MetB from Mycobacterium ulcerans / Acta Crystallogr F Struct Biol Commun 67:1154~1158
Berman, H. M / 2000 / The Protein Data Bank / Nucleic Acids Res 28:235~242
Chibata, I / 1957 / Studies on Amino Acids: V. Studies on the Enzymatic Resolution (IV). Enzymatic Resolution of DL-Methionine (2) VI. Studies on the Enzymatic Resolution (V). Enzymatic Resolution of DL-Lysine (2) VII. Studies on the Enzymatic Resolution (VI). A Survey of the Acylase in Molds VIII. Studies on the Enzymatic Resolution (VII). Specificity of Mold Acylase / Bull Agric Chem Soc Japan 21:291~307
Clausen, T / 1998 / Crystal structure of Escherichia coli cystathionine g-synthase at 1. 5 Å / EMBO J 17:6827~6838
Clifton, M. C / 2011 / Structue of the cystathionine g-synthase MetB from Mycobacterium ulcerans / Acta Crystallogr F Struct Biol Commun 67:1154~1158
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