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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.10
- 수록면
- 259 - 270 (12page)
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Flax contains very high levels of alpha-linolenic acid (57%) and a fatty acid desaturase 3-coding sequence (Lufad3) of flax has been amplified from the RNA isolated from developing seeds. The deduced amino acid sequence of LuFAD3 showed the presence of three histidine motifs, six membrane spanning domains and an endoplasmic reticulum (ER) retention signal KSK, indicating its plausible localization into the ER. Flax is not amenable for genetic transformation and not suitable for functional validation of Lufad3 gene. Hence, rice with well-developed genetic transformation has been selected as heterologous host system. Coding sequence of Lufad3 driven by maize Ubi1 promoter has been introduced into indica rice by Agrobacterium tumefaciens-mediated genetic transformation.
Southern analysis of putative transformants (T0) revealed signals at variable lengths at [5 kbp indicating random integration of transgene into the genomes of different transformants. The Mendelian segregation observed for selectable marker gene hyg in both T1 and T2 generations confirmed stable inheritance and single-site integration of transgenes. As compared to untransformed control (UC), homozygous transgenic rice expressing Lufad3 showed higher levels of essential a-linolenic acid in leaves and seeds validating its functionality.
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참고문헌 (66)
참고문헌 신청
T Adarme-Vega / 2012 / Microalgal biofactories: a promising approach towards sustainable omega-3 fatty acid production / Microbial Cell Factories 11(1):96
C. M. Albert / 2005 / Dietary -Linolenic Acid Intake and Risk of Sudden Cardiac Death and Coronary Heart Disease / Circulation 112(21):3232~3238
Altschul SF / 1990 / Basic local alignment search tool / J Mol Biol 215:403~410
T. Anai / 2003 / Improvement of rice (Oryza sativa L.) seed oil quality through introduction of a soybean microsomal omega-3 fatty acid desaturase gene / Plant Cell Reports 21(10):988~992
C. Balachandran / 2008 / An ecofriendly approach to process rice bran for high quality rice bran oil using supercritical carbon dioxide for nutraceutical applications / Bioresource Technology 99(8):2905~2912
C. M. Albert / 2005 / Dietary -Linolenic Acid Intake and Risk of Sudden Cardiac Death and Coronary Heart Disease / Circulation 112(21):3232~3238
Altschul SF / 1990 / Basic local alignment search tool / J Mol Biol 215:403~410
T. Anai / 2003 / Improvement of rice (Oryza sativa L.) seed oil quality through introduction of a soybean microsomal omega-3 fatty acid desaturase gene / Plant Cell Reports 21(10):988~992
C. Balachandran / 2008 / An ecofriendly approach to process rice bran for high quality rice bran oil using supercritical carbon dioxide for nutraceutical applications / Bioresource Technology 99(8):2905~2912
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