지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Perilla is an oilseed crop cultivated in Korea since ancient times. Due to the high α-linolenic acid content in perilla, perilla seed oil can easily become rancid. α-Linolenic acid is synthesized by two enzymes, endoplasmic reticulum-localized Δ15 desaturase (FAD3) and chloroplast-localized Δ15 desaturase (FAD7) in vivo. In order to lower the α-linolenic acid content of the seed oil without disturbing plant growth, we tried to suppress the expression of only the FAD3 gene using RNA interference, whilst maintaining the expression of the FAD7 gene. Seventeen transgenic plants with herbicide (BastaTM) resistance were obtained by Agrobacterium-mediated transformation using hypocotyls of perilla plants. The transgenic plants were firstly confirmed by treatment with 0.3% (v/v) BastaTM herbicide, and the expression of FAD3 was measured by Northern blot analysis. The α-linolenic acid content was 10-20%, 30-40%, and 60% in two, seven, and three of the twelve T1 transgenic perilla plants which had enough seeds to be analyzed for fatty acid composition, respectively. Analysis of the fatty acid composition of T2 progeny seeds from T1 plants with the lowest α-linolenic acid content showed that the homozygous lines had 6-10% α-linolenic acid content and the heterozygous lines had 20-26% α-linolenic acid content. It is expected that the reduction in α-linolenic acid content in perilla seed oil will prevent rancidity and can be utilized for the production of high-value functional ingredients such as high γ-linolenic acid.
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참고문헌 (24)
참고문헌 신청
Alwine JC / 1977 / Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes / Proc Natl Acad Sci USA 74:5350~5354
Mohammad Asif / 2011 / Health effects of omega-3,6,9 fatty acids: Perilla frutescens is a good example of plant oils / Oriental Pharmacy and Experimental Medicine 11(1):51~59
Bilyeu K / 2011 / Novel FAD3 mutant allele combinations produce soybeans containing 1% linolenic acid in the seed oil / Crop Sci 51:259~264
Ciftci ON / 2012 / Lipid components of flax, perilla, and chia seeds / Eur J Lipid Sci Tech 114:794~800
Cloutier S / 2011 / SSR-based linkage map of flax (Linim usitatissimum L) and mapping of QTLs underlying fatty acid composition traits / Mol Breeding 28:437~451
Mohammad Asif / 2011 / Health effects of omega-3,6,9 fatty acids: Perilla frutescens is a good example of plant oils / Oriental Pharmacy and Experimental Medicine 11(1):51~59
Bilyeu K / 2011 / Novel FAD3 mutant allele combinations produce soybeans containing 1% linolenic acid in the seed oil / Crop Sci 51:259~264
Ciftci ON / 2012 / Lipid components of flax, perilla, and chia seeds / Eur J Lipid Sci Tech 114:794~800
Cloutier S / 2011 / SSR-based linkage map of flax (Linim usitatissimum L) and mapping of QTLs underlying fatty acid composition traits / Mol Breeding 28:437~451
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