Curcumin protects against the intestinal ischemia-reperfusion injury: involvement of the tight junction protein ZO-1 and TNF-α related mechanism

Shuying Tian; Ruixue Guo; Sichen Wei; Yu Kong; Xinliang Wei; Weiwei Wang; Xiaomeng Shi; Hongyu Jiang

추천

검색

자료유형: 학술저널

저자정보: Shuying Tian (Cangzhou Central Hospital) Ruixue Guo (Cangzhou Central Hospital) Sichen Wei (Cangzhou Central Hospital) Yu Kong (Cangzhou Central Hospital) Xinliang Wei (Cangzhou Central Hospital) Weiwei Wang (Cangzhou Central Hospital) Xiaomeng Shi (Cangzhou Central Hospital) Hongyu Jiang (Cangzhou Central Hospital)

저널정보: 대한약리학회 The Korean Journal of Physiology & Pharmacology The Korean Journal of Physiology & Pharmacology 제20권 제2호

발행연도: 2016.3

수록면: 147 - 152 (6page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Present study aimed to investigate the effect of curcumin-pretreatment on intestinal I/R injury and on intestinal mucosa barrier. Thirty Wistar rats were randomly divided into: sham, I/R, and curcumin groups (n=10). Animals in curcumin group were pretreated with curcumin by gastric gavage (200 mg/kg) for 2 days before I/R. Small intestine tissues were prepared for Haematoxylin & Eosin (H&E) staining. Serum diamine oxidase (DAO) and tumor necrosis factor (TNF)-α levels were measured. Expression of intestinal TNF-α and tight junction protein (ZO-1) proteins was detected by Western blot and/or immunohistochemistry. Serum DAO level and serum and intestinal TNF-α leves were significantly increased after I/R, and the values were markedly reduced by curcumin pretreatment although still higher than that of sham group (p<0.05 or p<0.001). H&E staining showed the significant injury to intestinal mucosa following I/R, and curcumin pretreatment significantly improved the histological structure of intestinal mucosa. I/R insult also induced significantly down-regulated expression of ZO-1, and the effect was dramatically attenuated by curcumin-pretreatment. Curcumin may protect the intestine from I/R injury through restoration of the epithelial structure, promotion of the recovery of intestinal permeability, as well as enhancement of ZO-1 protein expression, and this effect may be partly attributed to the TNF-α related pathway.

#Curcumin #Intestine #Intestinal mucosa barrier #Intestinal permeability #Ischemia/reperfusion (I/R) injury #Tight junction protein (ZO-1)

참고문헌 (32)

참고문헌 신청

Berland T / 2008 / Acute mesenteric ischemia / Curr Gastroenterol Rep 10:341~346

Douzinas EE / 2003 / Myocardial ischemia in intestinal postischemic shock : the effect of hypoxemic reperfusion / Crit Care Med 31:2183~2189

Hassoun HT / 2001 / Post-injury multiple organ failure : the role of the gut / Shock 15:1~10

Mitic LL / 2000 / Molecular physiology and pathophysiology of tight junctions I. Tight junction structure and function : lessons from mutant animals and proteins / Am J Physiol Gastrointest Liver Physiol 279:250~254

Li Q / 2009 / Altered distribution of tight junction proteins after intestinal ischaemia/reperfusion injury in rats / J Cell Mol Med 13:4061~4076

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!