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논문 기본 정보

자료유형
학술저널
저자정보
Kwon Chang (R&D Center Cell Biotech Co. Ltd. Gimpo 10003 Republic of Korea) Kim Jong Won (R&D Center Cell Biotech Co. Ltd. Gimpo 10003 Republic of Korea) Park Young Kwang (R&D Center Cell Biotech Co. Ltd. Gimpo 10003 Republic of Korea) Kang Seungbeom (R&D Center Cell Biotech Co. Ltd. Gimpo 10003 Republic of Korea) Chung Myung Jun (R&D Center Cell Biotech Co. Ltd. Gimpo 10003 Republic of Korea) Kim Su Jeong (Highland Agriculture Research Institute National Institute of Crop Science Rural Development Admini) Lim Sanghyun (R&D Center Cell Biotech Co. Ltd. Gimpo 10003 Republic of Korea)
저널정보
한국미생물생명공학회 한국미생물·생명공학회지 한국미생물·생명공학회지 제51권 제1호
발행연도
2023.3
수록면
83 - 92 (10page)
DOI
10.48022/mbl.2302.02004

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초록· 키워드

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In this study, bioconversion of rutin to quercetin was confirmed by the fermentation of Korean indigenous probiotics and tartary buckwheat. Based on whole genome sequencing of 17 probiotics species, α-rhamnosidase, related to bioconversion of isoquercetin (quercetin 3-β-D glucoside) from rutin, is identified in the genome of CBT BG7, LC5, LR5, LP3, LA1, and LGA1. β-Glucosidase, related to bioconversion of isoquercetin to quercetin, is identified in the genome of all 17 species. Among the 17 probiotics species, 6 probiotics including CBT BG7, LR5, LP3, LA1, LGA1 and ST3 performed the bioconversion of rutin to quercetin up to 21.5 ± 0.3% at 7 days after fermentation. The fermentation of each probiotics together with enzyme complex Cellulase KN® was conducted to reduce the time of bioconversion. As a result, CBT LA1 which showed the highest yield of bioconversion of 21.5 ± 0.3% when the enzyme complex was not added showed high bioconversion yield of 84.6 ± 0.5% with adding the enzyme complex at 1 day after fermentation. In particular, CBT ST3 (96.2 ± 0.4%), SL6 (90.1 ± 1.4%) and LP3 (90.0 ± 0.4%) showed high yield of bioconversion more than 90%. In addition, such probiotics including high levels in quercetin indicated the inhibitory effects of NO production in LPS-induced RAW264.7 cells. In this study, we confirmed that the fermentation of Korean indigenous probiotics and enzyme complex together with roasted tartary buckwheat increased the content of quercetin and reduced the time of bioconversion of rutin to quercetin which is a bioactive compound related to anti-inflammatory, antioxidants, anti-obesity, and anti-diabetes.

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