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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한의용생체공학회 Biomedical Engineering Letters (BMEL) Biomedical Engineering Letters (BMEL) Vol.12 No.4
- 발행연도
- 2022.11
- 수록면
- 401 - 411 (11page)
- DOI
- https://doi.org/10.1007/s13534-022-00243-x
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For decades, the role of glial cells has attracted attention in the neuroscience field. Particularly, although the astrocyte is themost abundant glial cell type, it was believed to function as a passive support cell. However, recent evidence suggests thatastrocytes actively release various gliotransmitters and signaling entities that regulate the excitability of pre-and post-synapticneurons in the brain. In this study, we optimized the ratio of astrocytes and neurons to investigate the interaction betweenastrocytes and neurons. To this end, postnatal day 0–1 rodent hippocampi were dissociated and cultured. The neuron–astrocyteratio was monitored for up to 3 weeks after treating the cultures with 0, 1, and 5 μM of cytosine arabinoside (Ara-C)at DIV 2. Subsequently, from postnatal transgenic (TG) mouse expressing ChR2 on astrocytes, hippocampi were culturedon the microelectrode array (MEA) with the desired neuron–astrocyte ratio. The astrocyte was irradiated using a 473 nmblue laser for 30 s in a cycle of 10 Hz and electrophysiological recording was performed to verify the activities of neuronsinduced by the stimulated astrocytes. Astrocytes and neurons in both co-cultures increased at an identical ratio when treatedwith 1 μM Ara-C, whereas they decreased significantly when treated with 5 μM Ara-C. Particularly, the laser-stimulatedastrocytes induced an increase in the frequency of neuronal activities and lasted after illumination. The proposed co-cultureplatform is expected to be used in experiments to investigate the network between astrocytes and neurons in vitro.
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