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자료유형
학술저널
저자정보
Zhou Yin (College of Bioscience and Biotechnology Wuhan University of Bioengineering) Xu Zheng (College of Bioscience and Biotechnology Wuhan University of Bioengineering) Chen Xu (College of Bioscience and Biotechnology Wuhan University of Bioengineering) Zhou Junjiang (College of Bioscience and Biotechnology Wuhan University of Bioengineering) Wang Songtai (College of Bioscience and Biotechnology Wuhan University of Bioengineering) Xu Yanqin (College of Pharmacy Jiangxi University of Traditional Chinese Medicine)
저널정보
한국식물생명공학회 Plant Biotechnology Reports Plant Biotechnology Reports 제17권 제2호
발행연도
2023.4
수록면
243 - 253 (11page)
DOI
10.1007/s11816-022-00780-y

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Oriental orchids of the genus Cymbidium are famous for their flower fragrance and have great value in the horticultural mar- ket. To investigate the regulatory mechanisms of fragrance biosynthesis in Cymbidium faberi, the promoters of the CfAOC and CfJMT genes in the methyl jasmonate biosynthesis pathway were cloned and analyzed. Notably, the CfAOC promoter was transcriptionally active in all vegetative organs, which was not true for the CfJMT promoter, indicating that the latter is tissue-specific for the flowers of C. faberi. The dual-luciferase assay showed that the fragment between –629 and –1038 nt of the CfAOC promoter, as well as the fragment between –960 and –1121 nt of the CfJMT promoter, displayed specific interaction with the CfbHLH transcription factors. These results provide a theoretical basis for the genetic modification of the flower fragrance of Cymbidium based on specific CfbHLH transcription factors.

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