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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한미용성형외과학회 Archives of Aesthetic Plastic Surgery Archives of Aesthetic Plastic Surgery 제30권 제1호
- 발행연도
- 2024.1
- 수록면
- 16 - 21 (6page)
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초록· 키워드
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Background Adipose tissue-derived stem cells (ASCs) represent a rapidly evolving area of cell-based therapies. ASCs are harvested from the stromal vascular fraction (SVF), a heterogeneous mixture of ASCs and other cell types. Two methods are typically em ployed to obtain the SVF: enzymatic and mechanical. In this study, we examined the ef ficacy of ultrasonic cavitation in isolating the SVF from adipose tissue.
Methods Human adipose tissue was procured from three patients through an aesthet ic liposuction procedure. This tissue was then subjected to either an enzymatic method utilizing type II collagenase or an ultrasonic cavitation method using an Ultra Stemcell device. The cell counts and viability were determined using a cell counter. The immuno phenotype of the SVF was analyzed using real-time polymerase chain reaction.
Results Compared to the enzymatic method, the total cell count and cell viability of the SVF isolated through ultrasonic cavitation were relatively low. However, no significant difference was observed in the immunophenotype of CD45, CD11b, CD34, and CD105.
Conclusions The ultrasonic cavitation method constitutes a clinically practical approach that offers straightforward access in the operative field. This technique is a rapid and cost-effective method for isolating the SVF.
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