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논문 기본 정보

자료유형
학술저널
저자정보
한인화 (충남대학교) 조진아 (충남대 식품영양학과) 윤은성 (충남대 식품영양학과) 이수진 (충남대 식품영양학과) 전지향 (충남대학교) 정혜진 (충남대 식품영양학과) 홍지수 (충남대 식품영양학과) 김경아 (충남대학교)
저널정보
충북대학교 생활과학연구소 생활과학연구논총 생활과학연구논총 제28권 제2호
발행연도
2024.8
수록면
27 - 37 (11page)
DOI
10.36357/johe.2024.28.2.27

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초록· 키워드

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Background/Objectives: Blackcurrants are well-known for their high content of antioxidants and their anti-inflammatory properties, both of which contribute to various health benefits. This study aims to investigate the antioxidant and anti-inflammatory activities of 80% ethanol extracts of blackcurrant (BCEs), extracted using different methods. Methods: Blackcurrant powder was extracted by two different methods: stirring (BCE-S) and ultrasound (BCE-U). Antioxidant efficacy was assessed by measuring the total phenol content, total flavonoid content, and DPPH radical scavenging ability of the BCEs. Cell viability was determined using the WST assay in Raw 264.7 cells while the anti-inflammatory effect was evaluated by observing the expression levels of COX-2, an inflammation-related protein, through western blot analysis. Results: BCE-S and BCE-U did not exhibit significantly different total phenol content and DPPH radical scavenging ability. However, the total flavonoid content was higher in BCE-S than in BCE-U. Cytotoxicity evaluation revealed that both extracts did not show cytotoxicity at all treatment concentrations. The protein level of COX-2, increased by LPS treatment, was reduced in BCE-U but not in BCE-S. Despite the difference in total flavonoid content, there was no difference in antioxidant efficacy between the two extracts. However, BCE-U exhibited superior anti-inflammatory efficacy, suggesting variations occurred in the extract functionality based on the extraction method. Conclusion/Implications: These results provide fundamental data for establishing an efficient extraction method that can be tailored to obtain specific functionalities.

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