지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2003.6
- 수록면
- 45 - 50 (6page)
이용수
초록· 키워드
오류제보하기
Biological activities of PAHs are not known although PAHs are considered as carcinogens. Recent industrial society has human widely exposed to PAHs (polynuclear aromatic hydrocarbons) that are comming from the incomplete combustion of organic material as wider spread environmental contaminants. Our laboratory have been studied the effect of PAHs in the human breast cancer MCF-7 cells. In this study, we examined the human breast cancer MCF-7 cell as a new system to evaluate bioactivity of PAHs. We have selected 13 PARs to examine bioassay using CYP1A1-luciferase reporter gene expression system where CYP1A1 l.6 Kb Sflanking region DNA was cloned in front of luciferase reporter gene and this plasmid was transfected into MCF-7 cells transiently. This cells then used for the study to observe the effect of PAHs. We demonstrated that PARs induced the CYP1A1 promoter, CYPIAI mRNA and 7-ethoxyresolufin O-deethylao;e (EROD) activities in a concentration-dependant manner. None of PAHs that we have tested showed stronger stimulatory effect on CYP1 gene expression than TCDD. Benz(a)anthracene and benzo(b)tluoranthene were weak responders to CYP1A1 promoter activity stimulation, CYP1A1 mRNA and EROD induction in MCF-7 cells and these chemicals seemed to respond less either CYP1A1 mRNA or EROD than CYP1A1 promoter activity. Benzo(k)fluoranthene, chrysene, and dibenzo(a,h)anthracene showed strong response to CYP1A1 promoter activity stimulation, CYP1A1 mRNA increase and also EROD induction in MCF-7 cells. Results of dose response study suggested that two strong responding PAHs, such as benzo(k)fluoranthene and dibenzo(a,h )anthracene might be mediated through Aryl hydrocarbon receptors system in MCF-7 cells.
목차
ABSTRACT
Introduction
Materials and Methods
Results
Discussion
Acknowledgments
References
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Bioassays of Polycyclic Aromatic Hydrocarbons in ZR-75-1 Human Breast Cancer Cells
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Regulation of CYP1B1 Promotor Activity In MCF-7 Human Breast Cancer Cells
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Toxicological Research
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Effects of Polycyclic Aromatic Hydrocarbons on Liver and Lung Cytochrome P450s in Mice
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대한한의학회지
2014 .06
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CYP2E15B, CYP2E16, CYP2E17B, CYP2E12, and CYP2E13 Allele Frequencies in Iranian Populations
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ZR-75-1 human breast cancer cells to study the mechanism of action of PAHs
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Trichostatin A, a Histone Deacetylase Inhibitor Stimulate CYP3A4 Proximal Promoter Activity in Hepa-I Cells
Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea
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Cytochrome P-450 3A4 proximal promoter activity by histone deacetylase inhibitor in HepG2 cell.
한국응용약물학회 추계학술발표논문집
2003 .01
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Inhibition of 7-Alkoxyresorufin O-Dealkylation Activities of Recombinant Human CYP1A1 and CYP1B1 by Resveratrol
한국환경성돌연변이·발암원학회지
2002 .09
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생물정신의학
2000 .01
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UCI(KEPA) : I410-ECN-0101-2009-476-017705018