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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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Anticoagulant activities of a fermented edible brown alga, Laminaria ochotensis was investigated. L. ochotensis was fermented with 15% sugar (w/v) at 25℃ for 10 weeks. Anticoagulant activity was measured from the supernatant of algal mixture at biweekly intervals up to 10<SUP>th</SUP> week by activated partial thromboplastin (APTT), prothrombin time (PT) and thrombin time (TT) assay using citrated human plasma. Sample having high APTT activity (6<SUP>th</SUP> week) was filtered, ethanol precipitated and freeze-dried. The polysaccharide compound having anticoagulant activity was purified by DEAE ion exchange chromatography followed by Sepharose-4B gel filtration chromatography. Anticoagulant activity, polysaccharide concentration, and heparin like activity were determined for the collected fractions by APTT, phenol-H₂SO₄, and glycosaminoglycan assay, respectively. The anticoagulant activity assay showed that the activity was increased up to 6<SUP>th</SUP> week, and decreased thereafter. The concentration of our purified compound was 31.0 ㎍/㎖ and showed higher APTT activity than commercial heparin. At the same concentration of 31.0 ㎍/㎖, the heparin showed 186.5 sec activity while our purified compound showed an activity of 386 sec. Single spot on agarose gel electrophoresis showed that the compound was purified and polyacrylamide gel electrophoresis (PAGE) results revealed that the molecular mass of the purified polysaccharide compound was between 60 and 500 kDa. Therapeutic interest of the algal polysaccharide as an anticoagulant has recently been in highlighted. This purified anticoagulant compound from fermented L. ochotensis can be used as a model for anticoagulant agent or could be developed as an anticoagulant agent. This study can be extended to identify the structure and chemical composition of the purified polysaccharide, and to establish a relationship between structure and the function of the identified anticoagulant compounds.
목차
Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgement
References
참고문헌 (27)
참고문헌 신청
[학술지(정기간행물)] - Abdel-Fattah, A. F / 1974 / Studies of the purification and some properties of sargassan, a sulfated heteropolysaccharide from Sargassum linifolium / Carbohydr. Res 33 : 9 ~ 17
[학술지(정기간행물)] - Albuquerque, I. R. L / 2004 / Heterofucans from Dictyota menstrualis have anticoagulant activity / Braz. J. Med.Biol. Res 37 : 167 ~ 171
[기타자료] - Atlas, R. M / 1995 / Cellular metabolism: The generation of ATP book, St. Louis, pp. 147−154 / Principles of Microbiology. Mosby-Year
[단행본] - Chaplin, M. F / 1994 / Monosachcharides ; Carbohydrate analysis / Information Press Ltd., Oxford : 1 ~ 40
[학술지(정기간행물)] - Chargaff, E / 1936 / Studies on the chemistry of blood coagulation. II. On the inhibition of blood clotting by substances of high molecular weight / J. Biol. Chem 115 : 155 ~ 161
[학술지(정기간행물)] - Albuquerque, I. R. L / 2004 / Heterofucans from Dictyota menstrualis have anticoagulant activity / Braz. J. Med.Biol. Res 37 : 167 ~ 171
[기타자료] - Atlas, R. M / 1995 / Cellular metabolism: The generation of ATP book, St. Louis, pp. 147−154 / Principles of Microbiology. Mosby-Year
[단행본] - Chaplin, M. F / 1994 / Monosachcharides ; Carbohydrate analysis / Information Press Ltd., Oxford : 1 ~ 40
[학술지(정기간행물)] - Chargaff, E / 1936 / Studies on the chemistry of blood coagulation. II. On the inhibition of blood clotting by substances of high molecular weight / J. Biol. Chem 115 : 155 ~ 161
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