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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국식품영양과학회 Journal of Food Science and Nutrition Journal of Food Science and Nutrition Vol.11 No.3
- 발행연도
- 2006.9
- 수록면
- 191 - 197 (7page)
이용수
초록· 키워드
오류제보하기
Matrix metalloproteinase-2 (MMP-2) is a key enzyme involved in tumor invasiveness. The plant of Magnolia officinalis Rehd. et Wils. is often included as an ingredient in various herbal remedies recommended for cancer theraphies in Korea. Various extracts prepared from stems of M. officinalis were tested for cytotoxic activity on human hepatocellular carcinoma cell line, SK-Hep cells using the XTT assay method. Then, the inhibitory effect was examined on MMP-2 activity using gelatin zymography. Methanol (MeOH) extract of M. officinalis caused the strongest inhibition of the MMP-2 activity, as measured by gelatin zymography method for enzyme activity. IC?? values of fractions on MMP-2 activity were in a range of 4.9~11.3 ㎍/mL. Among each fraction, butanol and ethylacetate (EtOAc) fractions showed the strong inhibitory activities (IC??=10.7 and 4.9 ㎍/mL, respectively). When the M. officinalis’s constituents such as magnolol, honokiol, (-)-epigallocatechin gallate (EGCG) and ovovatol were examined for inhibitory effects on MMP-2 activity, EGCG showed strong inhibitory activity. However, MeOH extract of M. officinalis was dose-dependently inhibited to MMP-2 activity. The MeOH extract, hexane and EtOAc fractions (IC?? of ＞200 ㎍/mL) exhibited weak cytotoxicity activity, while butanol (IC??=80 ㎍/mL) and chloroform fractions (IC??=90 ㎍/mL) exhibited relatively strong cytotoxic activity. From these results, M. officinalis could be suitable for cancer treatment and chemopreventive drugs.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
ACKNOWLEDGMENTS
REFERENCES
참고문헌 (33)
참고문헌 신청
Cawston TE. 1996. Matrix metalloproteinase inhibitors and the prevention of connective tissue breakdown. Pharmacol Ther 70: 163-182.
Hiraoka NE, Allen IJ, Apel MR, Weiss SJ. 1998. Matrix metalloproteinases regulateneovascularization by acting as pericellular fibrinolysins. Cell 95: 365-377.
Liotta LA, Tryggvaason K, Garbisa S, Hart I, Foltz CM, Shafie S. 1980. Metastatic potential correlates with enzymatic degradation of basement membrane collagen. Nature 284: 67-68.
Shima IY, Sasaguri J, Kusukawa H, Yamana H, Fujita T, Kakegawa, Morimatsu M. 1992. Production of matrix metalloproteinase-2 and metalloproteinase-3 related to malignant behavior of esophageal carcinoma. A clinicopathologic study. Cancer 70: 2747-2753.
Tokuraku MH, Sato S, Murakami Y, Okada Y, Seiki M. 1995. Activation of the precursor of gelatinase A/72 kDa type IV collagenase/MMP-2 in lung carcinomas correlates with the expression of membrane-type matrix metalloproteinase (MT-MMP) and with lymph node
Hiraoka NE, Allen IJ, Apel MR, Weiss SJ. 1998. Matrix metalloproteinases regulateneovascularization by acting as pericellular fibrinolysins. Cell 95: 365-377.
Liotta LA, Tryggvaason K, Garbisa S, Hart I, Foltz CM, Shafie S. 1980. Metastatic potential correlates with enzymatic degradation of basement membrane collagen. Nature 284: 67-68.
Shima IY, Sasaguri J, Kusukawa H, Yamana H, Fujita T, Kakegawa, Morimatsu M. 1992. Production of matrix metalloproteinase-2 and metalloproteinase-3 related to malignant behavior of esophageal carcinoma. A clinicopathologic study. Cancer 70: 2747-2753.
Tokuraku MH, Sato S, Murakami Y, Okada Y, Seiki M. 1995. Activation of the precursor of gelatinase A/72 kDa type IV collagenase/MMP-2 in lung carcinomas correlates with the expression of membrane-type matrix metalloproteinase (MT-MMP) and with lymph node
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목련(Magnolia) 속
조경수
2005 .01
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UCI(KEPA) : I410-ECN-0101-2009-511-017343054