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[학술대회자료]

  • 학술대회자료

Y.K. Park M. Ikegaki M.H. Koo

UCI(KEPA) : I410-ECN-0101-2009-527-014645757

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초록

Three hundred samples of propolis were collected from various locations in Southern and Southeastern Brazil and analyscd by UV-spectrophotometer, RP-HPTLC and RP-HPLC. The samples were classified in accordance with patterns of UV-absorption spectra and RP-HPTLC and it was found that all samples were consisted of 7 kinds of propolis. Among these samples, one sample was performed further investigation, using water and ethanolic extracts of propolis.
Extraction of propolis was performed by using the water and various concentrations of ethanol as solvent. The extract was investigated hy measurement of absorption spectrum with a UV-spectrophotometer, reversed phase-high performance thin-layer chromatography, reversed phase-HPLC. Maximum absorption spectrum of all extracts was 290 ㎚, resembling flavonoid compounds, and the 80% ethanolic extract showed highest absorption at 290 ㎚. The most isosakuranetin, quercetin and kaempferol were extracted from mixtures of propolis and 60% ethanol, whereas 70% ethanol extracted the most pinocembrin and sakuranetin, but 80% ethanol extracted more kaempferide, acacetin, and isorhamnetin from propolis. The 60 to 80% ethanolic extracts of propolis inhibited highly microbial growth and 70 and 80% ethanolic extracts showed the greatest antioxidant activity and 80% ethanolic extract inhibited highly 10 hyaluronidase activity.
Propolis is bee glue, collected by bees from various plant sources. Bees mix the original propolis with beeswax and β-glucosidase which they secrete during the propoli. collection. This hydrolyzes the flavonoid glycosides into flavonoid aglycones. The propolis is used by bees as a glue to seal the opening of the hives and to eliminate outside invaders (1,2,3). The word propolis was derived from the Greek Pro- for defense and polis-for city, that is, defense of the city or thc hive.
So far, mainly polyphenolic compounds have been identified in the propolis collected by Apis mellifera, The main polyphcnols are flavonoids, accompanied by phenolic acids and their esters, phenolic aldehydes, alcohols, and ketones (4). Recently, four Di-O-caffeoyl quinic acid derivative were isolated from propolis and found that these compounds were potent hepatoprotective agents (5). It was also reported that extracts of propolis contained some compounds (not identified) which showed potent cytotoxity, having ED50, values equal to or less than 10 ㎍/mL, using human HT-1080 fibrosarcoma and murine colon 26-L5 carcinoma cells. (6). It was known that the ethanolic extracts of propolis exhibit various pharmacological activities such as antibacterial, antiviral, antifungal, anaesthetic, anti-inflammatory, hypotensive. immunostimulatory, and cytostatic properties (7,8). Therefore, the medical applications of propolis preparations have led to an increased interest in its chemical composition as well as to its origin.
Recently, the propolis preparations have heen used for the produclions of health foods and cosmetics. Tbese propolis preparations are ethanolic extracts of propolis and rarely water extracts.
On the other hand, we have noted thai quality and quantity of flavonoids aglycones of propolis were different Southeastern and Southern Braril. Consequently, we concluded that this is due to different plant ecologies (9,10). Later. we also found that the qualitative difference of flavonoids in propolis was not only upon plant ecology but the variety of the bees also related the difference of propolis compositions (11).
The objective of this presentation was to introduce relationship between some chemical compositions of propolis and variety of these bees and plant ecology. This presentation was also engaged in an adequate method for preparation of ethanolic extracts of propolis. The ethanolic extracts compared with water extract of propolis were evaluated their antimicrohial, antioxidant, anti-inflammatory, and antitumoral activities.

목차

Ⅰ. ABSTRACT
Ⅱ. MATERIALS AND METHODS
Ⅲ. RESULTS AND DISCUSSION
Ⅳ. REFERENCES
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