지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.3
- 수록면
- 75 - 85 (11page)
이용수
초록· 키워드
오류제보하기
Purpose: The purposes of this study are to examine the cytogenetic alterations of pancreatic cancer, by using fluorescent in situ hybridization (FISH), to determine thier correlation with the clinico-pathologic prognostic factors and to identify the cytogenetic factors that can predict the prognosis of pancreatic cancer.
Methods: Fresh frozen tissues of pancreatic cancer and normal pancreas that were obtained via pancreatic resection from 20 patients with pancreatic ductal adenocacinoma were analyzed by performing FISH with using locus-specific c-myc, p16, p53 probes and chromosome 18q, 20q probes. We cpmpared the FISH results with the clinico-pathologic prognostic factors. We also examined 16 paraffin-embedded tissues of pancreatic cancer by performing immunohistochemical staining (IHC) with monoclonal antibody to c-myc, p16, p53 and DPC. We then evaluated the correlation between the results of FISH and the results of IHC.
Results: At least one alteration of genes or chromosomes was detected in 18 (90.0%) of the 20 pancreatic cancer tissues by FISH, as compared with no alternation in the normal pancreatic tissues: these alteration were an increased copy number of c-myc (66.7%), a decreased copy number of p16 (70.6%), deletion of p53 (100%), loss of chromosome 18q (56.3%) and gain of chromosome 20q (45.0%). IHC demonstrated overexpression of c-myc and p53 in 31.3% and 50.0% of the pancreatic cancer specimens, respectively, and the loss of expressions of p16 and DPC in 25.0% and 93.3% of the pancreatic cancer specimens, respectively. The concordance rate of IHC with FISH was 33.3% to 61.5%. Analysis of the correlation between the cytogenetic changes identified by FISH or IHC and the pathologic prognostic factors showed that only chromosome 20q gain was significantly correlated with the histologic grade (p=0.098) and lymphovascular invasion (p=0.092). However there was no clinical correlation of the cytogenetic changes with respect to recurrence after operation.
Conclusion: This study confirms that most pancreatic cancers have cytogenetic alternations, as can be determined by FISH. Especially, the correlation between chromosome 20q gain and the prognostic pathologic factors offers the possibility of a new prognostic biologic marker located in chromosome 20q. However, further studies with more cases are needed to clarify the clinical significance of cytogenetic alternations in pancreatic cancer.
Methods: Fresh frozen tissues of pancreatic cancer and normal pancreas that were obtained via pancreatic resection from 20 patients with pancreatic ductal adenocacinoma were analyzed by performing FISH with using locus-specific c-myc, p16, p53 probes and chromosome 18q, 20q probes. We cpmpared the FISH results with the clinico-pathologic prognostic factors. We also examined 16 paraffin-embedded tissues of pancreatic cancer by performing immunohistochemical staining (IHC) with monoclonal antibody to c-myc, p16, p53 and DPC. We then evaluated the correlation between the results of FISH and the results of IHC.
Results: At least one alteration of genes or chromosomes was detected in 18 (90.0%) of the 20 pancreatic cancer tissues by FISH, as compared with no alternation in the normal pancreatic tissues: these alteration were an increased copy number of c-myc (66.7%), a decreased copy number of p16 (70.6%), deletion of p53 (100%), loss of chromosome 18q (56.3%) and gain of chromosome 20q (45.0%). IHC demonstrated overexpression of c-myc and p53 in 31.3% and 50.0% of the pancreatic cancer specimens, respectively, and the loss of expressions of p16 and DPC in 25.0% and 93.3% of the pancreatic cancer specimens, respectively. The concordance rate of IHC with FISH was 33.3% to 61.5%. Analysis of the correlation between the cytogenetic changes identified by FISH or IHC and the pathologic prognostic factors showed that only chromosome 20q gain was significantly correlated with the histologic grade (p=0.098) and lymphovascular invasion (p=0.092). However there was no clinical correlation of the cytogenetic changes with respect to recurrence after operation.
Conclusion: This study confirms that most pancreatic cancers have cytogenetic alternations, as can be determined by FISH. Especially, the correlation between chromosome 20q gain and the prognostic pathologic factors offers the possibility of a new prognostic biologic marker located in chromosome 20q. However, further studies with more cases are needed to clarify the clinical significance of cytogenetic alternations in pancreatic cancer.
목차
서론
방법
결과
고찰
결론
참고문헌
참고문헌 (25)
참고문헌 신청
1. [학술지(정기간행물)] - Conlon KC / 223 / Long-term survival after curative resection for pancreatic ductal adenocarcinoma. Clinicopathologic analysis of 5-year survivors / Ann Surg : 273 ~ 279
2. [학술지(정기간행물)] - Han SS / 2006 / Analysis of long-term survivors after surgical resection for pancreatic cancer / Pancreas 32 : 271 ~ 275
3. [학술지(정기간행물)] - Gorunova L / 1998 / Cytogenetic analysis of pancreatic carcinomas: intratumor heterogeneity and on random pattern of chromosome aberrations / Genes Chromosomes Cancer 23 : 81 ~ 99
4. [학술지(정기간행물)] - Griffin CA / 1995 / Consistent chromosome abnormalities in adenocarcinoma of the pancreas / Cancer Res 55 : 2394 ~ 2399
5. [학술지(정기간행물)] - Luttges J / 2001 / Allelic loss is often the first hit in the biallelic inactivation of the p53 and DPC4 genes during pancreatic carcinogenesis / Am J Pathol 158 : 1677 ~ 1683
2. [학술지(정기간행물)] - Han SS / 2006 / Analysis of long-term survivors after surgical resection for pancreatic cancer / Pancreas 32 : 271 ~ 275
3. [학술지(정기간행물)] - Gorunova L / 1998 / Cytogenetic analysis of pancreatic carcinomas: intratumor heterogeneity and on random pattern of chromosome aberrations / Genes Chromosomes Cancer 23 : 81 ~ 99
4. [학술지(정기간행물)] - Griffin CA / 1995 / Consistent chromosome abnormalities in adenocarcinoma of the pancreas / Cancer Res 55 : 2394 ~ 2399
5. [학술지(정기간행물)] - Luttges J / 2001 / Allelic loss is often the first hit in the biallelic inactivation of the p53 and DPC4 genes during pancreatic carcinogenesis / Am J Pathol 158 : 1677 ~ 1683
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Cancer Cytogenetics: Methodology Revisited
Annals of Laboratory Medicine
2014 .01
-
Cell Biology and Cytogenetics
대한두경부종양학회 학술대회
1998 .01
-
한국인 선천성 이상 질환이 의심되는 14,402 환자에 대한 세포유전학적 연구: YUMC의 31년 경험 (1974-2004년간)
Obstetrics & Gynecology Science
2005 .01
-
Does the Cytogenetic Analysis Using Fluorescence In Situ Hybridization Improve the Preoperative Diagnostic Accuracy of Pancreatic Ductal Adenocarcinoma?
Gut and Liver
2020 .01
-
37. Cytogenetic Analysis in Uterine Cervical Cancer Cell Line
대한체질인류학회 학술대회 연제 초록
1990 .04
-
Cytogenetic evaluation of a patient with ring chromosome 9 presenting failure to thrive and developmental delay
Korean journal of pediatrics
2008 .01
-
자동영상장치를 이용한 세포유전 연구
Laboratory Animal Research
1995 .12
-
골수형성이상증후군에서 동반되는 염색체 이상과 임상적 의미
대한내과학회지
2009 .01
-
자폐장애 환자의 세포유전학적 분석
소아청소년정신의학
1994 .12
-
췌장암의 임상적 고찰
Annals of Surgical Treatment and Research
1992 .02
-
Molecular and Cytogenetic Findings in 46, XX Males
대한생식의학회 학술대회
1996 .01
-
췌장암의 외과적 처치
Annals of Surgical Treatment and Research
1990 .04
-
췌장암 치료의 시대적 변화상
Annals of Surgical Treatment and Research
1995 .03
-
Prognostic Implication of Inflammation-Based Prognostic Scores in Patients with Pancreatic Cancer Treated with Pancreatic Surgery
대한외과학회 학술대회 초록집
2018 .11
-
염색체 핵형분석을 위한 세포유전학적 연구(Ⅱ)
Laboratory Animal Research
1992 .06
-
염색체 핵형분석을 위한 세포유전학적 연구(Ⅰ)
Laboratory Animal Research
1992 .06
-
FISH를 이용한 다운증후군과 에드워드증후군의 신속한 산전확인 : 309예의 임상적 고찰
Journal of genetic medicine
2007 .01
-
다운증후군의 세포유전학적 연구
대한불임학회잡지
1997 .01
-
Prenatal diagnosis of an unbalanced translocation between chromosome Y and chromosome 15 in a female fetus
Journal of genetic medicine
2016 .01
-
Combined Study of Cytogenetics and Fluorescence in Situ Hybridization (FISH) Analysis in Childhood Acute Lymphoblastic Leukemia (ALL) in a Tertiary Cancer Centre in South India
Asian Pacific journal of cancer prevention : APJCP
2012 .01
이 논문의 저자 정보
이 논문과 함께 이용한 논문
탄소나노튜브 탄소나노섬유의 수소분위기 처리에 따른 기계적 특성 비교
장훈식
,
전상구
,
백운봉
외 1명
대한기계학회 춘추학술대회
2018
.04
불가능을 가능하게하는 초정밀 냉간단조 순송공법
안학수
단조 심포지엄
2014
.06
3차원 유한요소법을 적용한 냉간단조 기어 성형 해석 및 설계
송종호
,
김수영
,
임용택
한국소성가공학회 학술대회 논문집
2002
.05
최적화기법의 적용을 통한 냉간단조품의 성형공정 평가
여홍태
,
박건형
,
허관도
소성·가공
2006
.04
냉간단조된 후판형 플랜지 돌출부 굽힘성형 공정연구
김대원
,
신영철
,
최호준
외 3명
소성·가공
2012
.11
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2014-514-001248295