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논문 기본 정보

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학술저널
저자정보
Jung-Ran Noh (한국생명공학연구원) Gil-Tae Gang (한국생명공학연구원) Yong-Hoon Kim (한국생명공학연구원) Keum-Jin Yang (한국생명공학연구원) Chul-Ho Lee (한국생명공학연구원) O-Su Na (하이테크산업개발연구원) Gi-Ju Kim (하이테크산업개발연구원) Won-Keun Oh (조선대학교) Young-Don Lee (제주대학교)
저널정보
대한지역사회영양학회 Nutrition Research and Practice Nutrition Research and Practice Vol.4 No.1
발행연도
2010.2
수록면
3 - 10 (8page)

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This study was performed to investigate the effect of desalinated underground seawater (named as ‘magma seawater’, MSW) of Jeju Island in Korea on lipid metabolism and antioxidant activity. MSW was collected from underground of Han-Dong in Jeju Island, and freely given to high fat diet (HFD)-fed C57BL/6 mice for 10 weeks. Although there were no significant differences in the body weight changes and plasma lipid levels, hepatic triglyceride levels were significantly lower in the MSW group than in the normal tap water (TW)-drunken control group. Furthermore, the activity of fatty acid synthase (FAS) was significantly decreased and carnitine palmitoyltransferase (CPT) activity was increased in MSW group compared to TW group. Similarly, real-time PCR analysis revealed that mRNA expressions of lipogenic genes were lowered in MSW groups compared to the control group. In a morphometric observation on the liver tissue, accumulation of fats was remarkably reduced in MSW group. Meanwhile, in vitro assay, free radical scavenging activity measured by using diphenylpicrylhydrazyl (DPPH) was increased in MSW group. The 2'-7'-dichlorofluorescein diacetate (DCF-DA) staining followed with fluorescent microscopy showed a low intensity of fluorescence in MSW-treated HepG2 cells, compared to TW-treated HepG2 cells, which indicated that the production of reactive oxygen species by tert-butyl hydroperoxide (t-BHP) in HepG2 cells was decreased by MSW treatment. The antioxidant effect of MSW on t-BHP-induced oxidative stress in HepG2 cells was supported by the increased activities of intracellular antioxidant enzymes such as catalase and glutathione reductase. From these results, we speculate that MSW has an inhibitory effect on lipogenesis in liver and might play a protective role against cell damage by t-BHP-induced oxidative stress.

목차

Abstract
Introduction
Materials and Methods
Results
Discussion
Acknowledgment
References

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