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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.12
- 수록면
- 219 - 229 (11page)
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N-myc downstream regulated gene 2 (NDRG2) is a member of the NDRG gene family. Our previous report indicated a possible role for NDRG2 in regulating the cytokine, interleukin-10 (IL-10), which is an important immunosuppressive cytokine. Several pathways, including p38-MAPK, NF-κB , and JAK/STAT, are used for IL-10 production, and the JAK/STAT pathway can be inhibited in a negative feedback loop by the inducible protein, SOCS3. In the present study, we investigated the effect of NDRG2 gene expression on IL-10 signaling pathway that is modulated via SOCS3 and STAT3. Methods: We generated NDRG2-overexpressing U937 cell line (U937-NDRG2) and treated the cells with PMA to investigate the role of NDRG2 in IL-10 production. U937 cells were also transfected with SOCS3- or NDRG2-specific siRNAs to examine whether the knockdown of SOCS3 or NDRG2 influenced IL-10 expression. Lastly, STAT3 and SOCS3 induction was measured to identify the signaling pathway that was associated with IL-10 production. Results: RT-PCR and ELISA assays showed that IL-10 was increased in U937-mock cells upon stimulation with PMA, but IL-10 was inhibited by overexpression NDRG2. After PMA treatment, STAT3 phosphorylation was decreased in a time-dependent manner in U937-mock cells, whereas it was maintained in U937-NDRG2 cells. SOCS3 was markedly reduced in U937-NDRG2 cells compared with U937-mock cells. IL-10 production after PMA stimulation was reduced in U937 cells when SOCS3 was inhibited, but this effect was less severe when NDRG2 was inhibited. Conclusion: NDRG2 expression modulates SOCS3 and STAT3 activity, eventually leading to the inhibition of IL-10 production.
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참고문헌 (43)
참고문헌 신청
1. [학술지(정기간행물)] - Kim YJ / 2009 / NDRG2 suppresses cell proliferation through down-regulation of AP-1 activity in human colon carcinoma cells / Int J Cancer 124 : 7 ~ 15
2. [학술지(정기간행물)] - Choi SC / 2003 / Expression and regulation of NDRG2 (N-myc downstream regulated gene 2) during the differentiation of dendritic cells / FEBS Lett 553 : 413 ~ 418
3. [학술지(정기간행물)] - Park Y / 2007 / SOCS1 induced by NDRG2 expression negatively regulates STAT3 activation in breast cancer cells / Biochem Biophys Res Commun 363 : 361 ~ 367
4. [학술지(정기간행물)] - Liu N / 2008 / N-Myc downstream-regulated gene 2 is involved in p53-mediated apoptosis / Nucleic Acids Res 36 : 5335 ~ 5349
5. [학술지(정기간행물)] - O'Garra A / 2008 / Strategies for use of IL-10 or its antagonists in human disease / Immunol Rev 223 : 114 ~ 113
2. [학술지(정기간행물)] - Choi SC / 2003 / Expression and regulation of NDRG2 (N-myc downstream regulated gene 2) during the differentiation of dendritic cells / FEBS Lett 553 : 413 ~ 418
3. [학술지(정기간행물)] - Park Y / 2007 / SOCS1 induced by NDRG2 expression negatively regulates STAT3 activation in breast cancer cells / Biochem Biophys Res Commun 363 : 361 ~ 367
4. [학술지(정기간행물)] - Liu N / 2008 / N-Myc downstream-regulated gene 2 is involved in p53-mediated apoptosis / Nucleic Acids Res 36 : 5335 ~ 5349
5. [학술지(정기간행물)] - O'Garra A / 2008 / Strategies for use of IL-10 or its antagonists in human disease / Immunol Rev 223 : 114 ~ 113
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