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논문 기본 정보

자료유형
학술저널
저자정보
Sararat Monkhung (Silpakorn University) Yun-Tae Kim (Future Agricultural Strategy Institute) Yong-Seong Lee (Chonnam National University) Jeong-Yong Cho (Mokpo National University) Jae-Hak Moon (Chonnam National University) Kil-Yong Kim (Chonnam National University)
저널정보
한국토양비료학회 한국토양비료학회지 한국토양비료학회지 제49권 제4호
발행연도
2016.8
수록면
393 - 400 (8page)

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초록· 키워드

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Fusarium graminearum is the main cause of substantial economic loss in wheat production. The aim of this study is to investigate biocontrol potential of Lysobacter antibioticus HS124 against F. graminearum and to purify an antifungal compound. In preliminary study, n-butanol crude extract revealed destructive alterations in the hyphal morphology of F. graminearum and almost degraded with 1,000 μg mL<SUP>-1</SUP> concentration. For further study, the antifungal compound extracted from the n-butanol crude extract of L. antibioticus HS124 was identified as N-Butyl-tetrahydro-5-oxofuran-2-carboxamide (C<SUB>9</SUB>H<SUB>16</SUB>NO₃) using NMR (<SUP>1</SUP>H–NMR, <SUP>13</SUP>C–NMR,<SUP>1</SUP>H–<SUP>1</SUP>H COSY, HMBC, and HMQC), and HR-ESI-MS analysis. To our knowledge, N-Butyl-tetrahydro-5-oxofuran-2-carboxamide may be a novel compound with molecular weight of 186.1130. The minimum inhibitory concentration value of antifungal compound was 62.5 μg mL<SUP>-1</SUP> against F. graminearum. In an in vivo pot experiment, crown rot disease from F. graminearum was inhibited when wheat seeds were treated with both HS124 culture and F. graminearum. Growth of wheat seedling was enhanced by treatment of HS124 compared to control. Our results suggest that L. antibioticus HS124 characterized in this study could be successfully used to control F. graminearum and could be used as an alternative to chemical fungicides in modern agriculture.

목차

Introduction
Materials and Methods
Results and Discussion
References

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UCI(KEPA) : I410-ECN-0101-2017-523-001137256