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지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.8
- 수록면
- 431 - 437 (7page)
이용수
초록· 키워드
오류제보하기
The study of antigenic epitopes from Toxoplasma gondii has not only enhanced our understanding of the structure and function of antigens, the reactions between antigens and antibodies, and many other aspects of immunology, but it also plays a significant role in the development of new diagnostic reagents and vaccines. In the present study, T. gondii GRA6 epitopes were identified using bioinformatics tools and a synthetic peptide technique. The potential B cell epitopes of GRA6 predicted by bioinformatics tools concentrated upon 3 regions of GRA6, 1-20 aa, 44-103 aa, and 172-221 aa. Ten shorter peptides from the 3 regions were synthesized and assessed by ELISA using pig sera from different time points after infection. Three of the 10 peptides (amino acids 44-63, 172-191, and 192-211) tested were recognized by all sera and determined to be immunodominant B-cell epitopes of GRA6. The results indicated that we precisely and accurately located the T. gondii GRA6 epitopes using pig sera collected at different time points after infection. The identified epitopes may be very useful for further studies of epitope-based vaccines and diagnostic reagents.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (30)
참고문헌 신청
[학술지(정기간행물)] - Dubey JP / 1994 / Persistence of immunity to toxoplasmosis in pigs vaccinated with a non-persistent strain of Toxoplasma gondii / Am J Vet Res 55 : 982 ~ 987
[학술지(정기간행물)] - Ortega-Pacheco A / 2011 / Infection dynamic of Toxoplasma gondii in two fattening pig farms exposed to high and low cat density in an endemic region / Vet Parasitol 175 : 367 ~ 371
[학술지(정기간행물)] - Wang YH / 2013 / Immunization with excreted-secreted antigens reduces tissue cyst formation in pigs / Parasitol Res 112 : 3835 ~ 3842
[학술지(정기간행물)] - Cong H / 2008 / Multi-epitope DNA vaccine linked to the A2/B subunit of cholera toxin protect mice against Toxoplasma gondii / Vaccine 26 : 3913 ~ 3921
[학술지(정기간행물)] - Darcy F / 1992 / Protection of mice and nude rats against toxoplasmosis by a multiple antigenic peptide construction derived from Toxoplasma gondii P30 antigen / J Immunol 149 : 3636 ~ 3641
[학술지(정기간행물)] - Ortega-Pacheco A / 2011 / Infection dynamic of Toxoplasma gondii in two fattening pig farms exposed to high and low cat density in an endemic region / Vet Parasitol 175 : 367 ~ 371
[학술지(정기간행물)] - Wang YH / 2013 / Immunization with excreted-secreted antigens reduces tissue cyst formation in pigs / Parasitol Res 112 : 3835 ~ 3842
[학술지(정기간행물)] - Cong H / 2008 / Multi-epitope DNA vaccine linked to the A2/B subunit of cholera toxin protect mice against Toxoplasma gondii / Vaccine 26 : 3913 ~ 3921
[학술지(정기간행물)] - Darcy F / 1992 / Protection of mice and nude rats against toxoplasmosis by a multiple antigenic peptide construction derived from Toxoplasma gondii P30 antigen / J Immunol 149 : 3636 ~ 3641
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UCI(KEPA) : I410-ECN-0101-2017-513-001124531