2-DE and MALDI-TOF MS-based identification of bovine whey proteins in milk collected soon after parturition

Jae Eun Lee; Tao Lin; Jung Won Kang; Hyun Young Shin; Joo Bin Lee; Dong Il Jin

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자료유형: 학술저널

저자정보: Jae Eun Lee (Chungnam National University) Tao Lin (Chungnam National University) Jung Won Kang (Chungnam National University) Hyun Young Shin (Chungnam National University) Joo Bin Lee (Chungnam National University) Dong Il Jin (Chungnam National University)

저널정보: 충남대학교 농업과학연구소 Korean Journal of Agricultural Science Korean Journal of Agricultural Science Vol.45 No.4

발행연도: 2018.12

수록면: 635 - 643 (9page)

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Bovine milk is widely consumed by humans and is a primary ingredient of dairy foods. Proteomic approaches have the potential to elucidate complex milk proteins and have been used to study milk of various species. Here, we performed a proteomic analysis using 2-dimensional electrophoresis (2-DE) and matrix assisted laser desorption ionization.time of flight mass spectrometer (MALDI-TOF MS) to identify whey proteins in bovine milk obtained soon after parturition (bovine early milk). The major casein proteins were removed, and the whey proteins were analyzed with 2.dimensional polyacrylamide gel electrophoresis (2-D PAGE). The whey proteins (2 mg) were separated by pI and molecular weight across pH ranges of 3.0 - 10.0 and 4.0 - 7.0. The 2-DE gels held about 300 to 700 detectable protein spots. We randomly picked 12 and nine spots that were consistently expressed in the pH 3.0 - 10.0 and pH 4.0 - 7.0 ranges, respectively. Following MALDI-TOF MS analysis, the 21 randomly selected proteins included proteins known to be present in bovine milk, such as albumin, lactoferrin, serum albumin precursor, T cell receptor, polymeric immunoglobulin receptor, pancreatic trypsin inhibitor, aldehyde oxidase and microglobulin. These proteins have major functions in immune responses, metabolism and protein binding. In summary, we herein identified both known and novel whey proteins present in bovine early milk, and our sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) analysis revealed their expression pattern.

#2-DE #bovine early milk #MALDI-TOF #whey proteomics

참고문헌 (25)

참고문헌 신청

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